MFG-E8 Antibody

Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining in human heart muscle and liver tissues.. Corresponding MFGE8 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in intercalated discs.

Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human kidney shows moderate to strong cytoplasmic positivity in aortic media.

Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Discontinued

MFG-E8 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LKNNSIPDKQITASSSYKTWGLHLFSWNPSYARLDKQGNFNAWVAGSYGNDQWLQVDLGSSKEVTGIITQGARNFGSVQFVASYKVAYSNDSANWTEYQDPRTGSSKIFPGNWDNHSHKKNLFETPILARYVRILPVAWHNRIALRLEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MFGE8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MFG-E8 Antibody

• BA46
• Breast epithelial antigen BA46
• EDIL1
• hP47
• HsT19888
• lactadherin
• Lactahedrin
• Medin
• MFG1
• MFGE8
• MFG-E8
• MFGM
• milk fat globule-EGF factor 8 protein
• Milk fat globule-EGF factor 8
• O-acetyl disialoganglioside synthase
• OAcGD3S
• SED1
• SPAG10
• sperm associated antigen 10
• sperm surface protein hP47

Background

Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MFG-E8 Antibody (NBP1-90023)(2)

Publications using NBP1-90023

• Wu Y, Wang F, Shi J et al. CircSMAD2 accelerates endometrial cancer cell proliferation and metastasis by regulating the miR-1277-5p/MFGE8 axis Journal of gynecologic oncology 2023-01-02 [PMID: 36659830] (IHC, Human)
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: MFGE8