MFG-E8 Antibody (SPM291)

Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with MFG-E8 Antibody (SPM291)

Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (SPM291)

Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MFG-E8 Antibody (SPM291).

Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Pancreas stained with MFG-E8 Antibody (SPM291)

• Reactivity: Hu
• Applications: IHC
• Clone: SPM291
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MFG-E8 Antibody (SPM291) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11482)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human milk fat globule membrane preparation (Uniprot: Q08431)
• Localization: Cell Surface & Cytoplasmic
• Marker: Breast Epithelial Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MFGE8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for MFG-E8 Antibody (SPM291)

• BA46
• Breast epithelial antigen BA46
• EDIL1
• hP47
• HsT19888
• lactadherin
• Lactahedrin
• Medin
• MFG1
• MFGE8
• MFG-E8
• MFGM
• milk fat globule-EGF factor 8 protein
• Milk fat globule-EGF factor 8
• O-acetyl disialoganglioside synthase
• OAcGD3S
• SED1
• SPAG10
• sperm associated antigen 10
• sperm surface protein hP47

Background

Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MFGE8
• Uniprot:
  • Q08431()
  • Q08431()