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MFG-E8 Antibody (MFG-06)

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Immunohistochemistry-Paraffin: MFG-E8 Antibody (MFG-06) [NBP2-44410] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MFG-E8 Antibody (MFG-06)
Immunohistochemistry-Paraffin: MFG-E8 Antibody (MFG-06) [NBP2-44410] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MFG-E8 Antibody (MFG-06)
Immunohistochemistry-Paraffin: MFG-E8 Antibody (MFG-06) [NBP2-44410] - Human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-06)
Immunohistochemistry-Paraffin: MFG-E8 Antibody (MFG-06) [NBP2-44410] - Formalin-fixed, paraffin-embedded human Pancreas stained with MFG-E8 Antibody (MFG-06)
Immunohistochemistry-Paraffin: MFG-E8 Antibody (MFG-06) [NBP2-44410] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with MFG-E8 Antibody (MFG-06)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
MFG-06
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
IHC-Prediluted
NBP2-48262
Azide and BSA Free
NBP3-11473
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MFG-E8 Antibody (MFG-06) Summary

Immunogen
Human milk fat globule membrane preparation (Uniprot: Q08431)
Localization
Cell Surface & Cytoplasmic
Marker
Breast Epithelial Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MFGE8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11473)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for MFG-E8 Antibody (MFG-06)

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Background

Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MFGE8
Uniprot