MFG-E8 Antibody (EDM45) - Azide and BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC-P, CyTOF-ready, ICC/IF
Clone
EDM45
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

MFG-E8 Antibody (EDM45) - Azide and BSA Free Summary

Immunogen
Delipidated human milk fat globule membrane preparation
Localization
Cell Surface & Cytoplasmic
Marker
Breast Epithelial Marker
Specificity
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MFGE8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • CyTOF-ready
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 15q25 Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for MFG-E8 Antibody (EDM45) - Azide and BSA Free

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MFGE8