Meprin alpha Subunit/MEP1A Antibody - BSA Free

Western Blot: Meprin alpha Subunit/MEP1A Antibody [NBP1-32595] - (30 ug whole cell lysate) A431 7.5% SDS PAGE; antibody diluted at 1:1000

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Meprin alpha Subunit/MEP1A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Meprin alpha Subunit/MEP1A. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MEP1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000
• Application Notes: IHC-P and ICC/IF reactivity reported in scientific literature (PMID: 23427141).
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23427141). Rat (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Meprin alpha Subunit/MEP1A Antibody - BSA Free

• bA268F1.1 (meprin A alpha (PABA peptide hydrolase))
• EC 3.4.24
• EC 3.4.24.18
• endopeptidase-2
• MEP1A
• meprin A subunit alpha
• meprin A, alpha (PABA peptide hydrolase)
• Meprin alpha Subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
• PABA peptide hydrolase
• PPH alpha
• PPHA

Background

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes and in leukocytes and certain cancer cells. Mature meprins are oligomers of evolutionarily related, separately encoded alpha and/or beta subunits. Homooligomers of meprin-alpha are secreted; oligomers containing meprin-beta are associated with the plasma membrane. Substrates include bioactive peptides and extracellular matrix proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Meprin alpha Subunit/MEP1A Antibody (NBP1-32595)(1)

Publication using NBP1-32595

• Kaushal GP, Haun RS, Herzog C, Shah SV. Meprin A metalloproteinase and its role in acute kidney injury. Am J Physiol Renal Physiol 2013-02-20 [PMID: 23427141] (IHC-P, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: MEP1A
• Entrez:
  • 4224(Human)
• Uniprot:
  • Q16819(Human)