Meprin beta Subunit/MEP1B Antibody

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Meprin beta Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Meprin beta Subunit/MEP1B was detected in perfusion fixed frozen sections of mouse intestine using 1 µg/mL Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of intestinal villi.View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Meprin beta Subunit/MEP1B Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Meprin beta Subunit/MEP1B
  Leu21-Ser594 (Thr75Ile, Ile432Val)
  Accession # Q61847
• Specificity: Detects mouse Meprin beta Subunit/MEP1B in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human MEP1B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEP1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin beta Subunit/MEP1B Antibody

• EC 3.4.24.63
• endopeptidase-2
• MEP1B
• meprin A subunit beta
• meprin A, beta
• Meprin B
• Meprin beta Subunit
• N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
• PABA peptide hydrolase
• PPH beta

Background

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as meprin A (composed of alpha subunits with or without beta subunits) and meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 704 amino acid sequence of mouse meprin beta subunit precursor consists of a signal peptide (residues 1-20), a pro region (residues 21-62), and a mature chain (residues 63-704) containing following domains, catalytic (residues 63-260), MAM (residues 261-430), MATH (residues 431-586), EGF-like (residues 607-647), transmembrane (residues 655-678), and cytoplasmic (residues 679-704). The pro enzyme terminating at residue 594 was expressed and the secreted protein purified from conditioned medium. The amino acid sequence has Ile and Val at position 75 and 432 instead of Thr and Ile, respectively. After trypsin treatment, the activated enzyme cleaved a flurogenic peptide, which contains Asp and Glu, the preferred residues found in the P1’ and P1 sites (3).

1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
2. Stocker, W. et al. (1995) Protein Sci. 4:823.
3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meprin beta Subunit/MEP1B Antibody (AF3300)(5)

Publications using AF3300

• Shaymaa Abousaad, Faihaa Ahmed, Ayman Abouzeid, Elimelda Moige Ongeri Meprin beta expression modulates the interleukin‐6 mediated JAK2‐STAT3 signaling pathway in ischemia/reperfusion‐induced kidney injury Physiological Reports 9/18/2022 [PMID: 36117389]
• V Biasin, M Wygrecka, LM Marsh, C Becker-Pau, L Brcic, B Ghanim, W Klepetko, A Olschewski, G Kwapiszews Meprin ? contributes to collagen deposition in lung fibrosis Sci Rep, 2017-01-06;7(0):39969. 2017-01-06 [PMID: 28059112] (IHC-P, Mouse)
• Christian Herzog, Raju Marisiddaiah, Randy S. Haun, Gur P. Kaushal Basement membrane protein nidogen-1 is a target of meprin beta in cisplatin nephrotoxicity Toxicology Letters 7/16/2015 [PMID: 25957482]
• Schutte A, Ermund A, Becker-Pauly C, Johansson M, Rodriguez-Pineiro A, Backhed F, Muller S, Lottaz D, Bond J, Hansson G Microbial-induced meprin beta cleavage in MUC2 mucin and a functional CFTR channel are required to release anchored small intestinal mucus. Proc Natl Acad Sci U S A, 2014-08-11;111(34):12396-401. 2014-08-11 [PMID: 25114233] (IHC, Mouse)
• Herzog C, Haun R, Ludwig A, Shah S, Kaushal G ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A. J Biol Chem, 2014-03-24;289(19):13308-22. 2014-03-24 [PMID: 24662289] (IHC-P, Western Blot, Mouse)

Review for Meprin beta Subunit/MEP1B Antibody (AF3300) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Western blot and immunofluorescence Mouse 08/20/2019

Summary

• Application: Other
• Sample Tested: C57BL/6J kidney,C57BL/6J urine
• Species: Mouse
• Lot: XQQ0116021

Comments

• Comments: 1:5000 for WB

Bioinformatics

• Gene Symbol: MEP1B
• Uniprot:
  • Q61847()