Meprin beta Subunit/MEP1B Antibody

Images

 
Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) ...read more
Meprin beta Subunit/MEP1B was detected in perfusion fixed frozen sections of mouse intestine using 1 µg/mL Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Meprin beta Subunit/MEP1B Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Meprin beta Subunit/MEP1B
Leu21-Ser594 (Thr75Ile, Ile432Val)
Accession # Q61847
Specificity
Detects mouse Meprin beta Subunit/MEP1B in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human MEP1B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MEP1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3300 in the following applications:

Publications
Read Publications using
AF3300 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin beta Subunit/MEP1B Antibody

  • EC 3.4.24.63
  • endopeptidase-2
  • MEP1B
  • meprin A subunit beta
  • meprin A, beta
  • Meprin B
  • Meprin beta Subunit
  • N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
  • N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
  • PABA peptide hydrolase
  • PPH beta

Background

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as meprin A (composed of alpha subunits with or without beta subunits) and meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 704 amino acid sequence of mouse meprin beta subunit precursor consists of a signal peptide (residues 1-20), a pro region (residues 21-62), and a mature chain (residues 63-704) containing following domains, catalytic (residues 63-260), MAM (residues 261-430), MATH (residues 431-586), EGF-like (residues 607-647), transmembrane (residues 655-678), and cytoplasmic (residues 679-704). The pro enzyme terminating at residue 594 was expressed and the secreted protein purified from conditioned medium. The amino acid sequence has Ile and Val at position 75 and 432 instead of Thr and Ile, respectively. After trypsin treatment, the activated enzyme cleaved a flurogenic peptide, which contains Asp and Glu, the preferred residues found in the P1’ and P1 sites (3).

  1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
  2. Stocker, W. et al. (1995) Protein Sci. 4:823.
  3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
  4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3220
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, ICFlow
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NBP2-92546
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-67717
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-33903
Species: Hu
Applications: IHC, IHC-P
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB600-930
Species: Hu, Rt
Applications: WB, ELISA
NBP1-46535
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NBP1-89993
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-41187
Species: Hu
Applications: WB, ELISA, IF
7270-IL/CF
Species: Hu
NBP1-90824
Species: Hu
Applications: WB, ICC/IF
NBP2-92677
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-56565
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF3300
Species: Mu
Applications: WB, IHC, IP

Publications for Meprin beta Subunit/MEP1B Antibody (AF3300)(3)

Review for Meprin beta Subunit/MEP1B Antibody (AF3300) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3300:
Filter by Applications
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 Meprin beta Subunit/MEP1B AF3300
Enlarge
reviewed by:
Anonymous
Mouse 08/20/2019
View

Summary

Sample TestedC57BL/6J urine,C57BL/6J kidney
SpeciesMouse
LotXQQ0116021

Comments

Comments1:5000 for WB

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Meprin beta Subunit/MEP1B Antibody (AF3300) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Meprin beta Subunit/MEP1B Products

Bioinformatics Tool for Meprin beta Subunit/MEP1B Antibody (AF3300)

Discover related pathways, diseases and genes to Meprin beta Subunit/MEP1B Antibody (AF3300). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Meprin beta Subunit/MEP1B Antibody (AF3300)

Discover more about diseases related to Meprin beta Subunit/MEP1B Antibody (AF3300).
 

Pathways for Meprin beta Subunit/MEP1B Antibody (AF3300)

View related products by pathway.

PTMs for Meprin beta Subunit/MEP1B Antibody (AF3300)

Learn more about PTMs related to Meprin beta Subunit/MEP1B Antibody (AF3300).

Blogs on Meprin beta Subunit/MEP1B

There are no specific blogs for Meprin beta Subunit/MEP1B, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/20/2019
Application:
Species: Mouse

Bioinformatics

Gene Symbol MEP1B
Uniprot