Meprin alpha Subunit/MEP1A Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Meprin alpha Subunit/MEP1A Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Meprin alpha Subunit/MEP1A
  Val34-Arg615
  Accession # NP_032611
• Specificity: Detects mouse Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant human MEP1A is observed and less than 5% cross‑reactivity with recombinant mouse MEP1B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEP1A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin alpha Subunit/MEP1A Antibody [Unconjugated]

• bA268F1.1 (meprin A alpha (PABA peptide hydrolase))
• EC 3.4.24
• EC 3.4.24.18
• endopeptidase-2
• MEP1A
• meprin A subunit alpha
• meprin A, alpha (PABA peptide hydrolase)
• Meprin alpha Subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
• PABA peptide hydrolase
• PPH alpha
• PPHA

Background

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or hetero-oligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 760 amino acid sequence of mouse meprin alpha  subunit precursor consists of a signal peptide (residues 1‑33), a pro region (residues 34‑77), and a mature chain (residues 78‑760) containing the following domains, catalytic (residues 78‑275), MAM (residues 276‑445), MATH (residues 447‑607), EGF-like (residues 684‑724), transmembrane (residues 727‑754), and cytoplasmic (residues 755‑760) (5). The pro enzyme terminating at residue 615 was expressed and the secreted protein purified from conditioned medium. The molecular masses of recombinant mouse MEP1A are similar to those observed for the alpha  subunit of rat Meprin A (6).

1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
2. Stocker, W. et al. (1995) Protein Sci. 4:823.
3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
5. Jiang, W. et al. (1992) J. Biol. Chem. 267:9185.
6. Bertenshaw, G.P. et al. (2003) J. Biol. Chem. 278:2522.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meprin alpha Subunit/MEP1A Antibody (AF4007)(3)

Publications using AF4007

• Jian-Bin Zhang, Meng-Ting Li, Shuang-Zhe Lin, Yu-Qing Cheng, Jian-Gao Fan, Yuan-Wen Chen Therapeutic Effect of Prolyl Endopeptidase Inhibitor in High-fat Diet-induced Metabolic Dysfunction-associated Fatty Liver Disease Journal of Clinical and Translational Hepatology 2023-10-28 [PMID: 37577240] (Western Blot, Mouse)
• Romero CA, Kumar N, Nakagawa P et al. Renal release of Ac-SDKP is part of an antifibrotic peptidergic system in the kidney. Am J Physiol Renal Physiol 11/7/2018 [PMID: 30403163]
• Christian Herzog, Raju Marisiddaiah, Randy S. Haun, Gur P. Kaushal Basement membrane protein nidogen-1 is a target of meprin beta in cisplatin nephrotoxicity Toxicology Letters 2015-07-16 [PMID: 25957482]

Bioinformatics

• Gene Symbol: MEP1A
• Uniprot:
  • NP_032611()