Meprin alpha Subunit/MEP1A Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

Meprin alpha Subunit/MEP1A Antibody [Alexa Fluor® 594] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Meprin α Subunit/MEP1A
  Val34-Arg615
  Accession # NP_032611
• Specificity: Detects mouse Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant human MEP1A is observed and less than 5% cross‑reactivity with recombinant mouse MEP1B is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin alpha Subunit/MEP1A Antibody [Alexa Fluor® 594]

• bA268F1.1 (meprin A alpha (PABA peptide hydrolase))
• EC 3.4.24
• EC 3.4.24.18
• endopeptidase-2
• MEP1A
• meprin A subunit alpha
• meprin A, alpha (PABA peptide hydrolase)
• Meprin alpha Subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
• PABA peptide hydrolase
• PPH alpha
• PPHA

Background

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or hetero-oligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 760 amino acid sequence of mouse meprin alpha  subunit precursor consists of a signal peptide (residues 1‑33), a pro region (residues 34‑77), and a mature chain (residues 78‑760) containing the following domains, catalytic (residues 78‑275), MAM (residues 276‑445), MATH (residues 447‑607), EGF-like (residues 684‑724), transmembrane (residues 727‑754), and cytoplasmic (residues 755‑760) (5). The pro enzyme terminating at residue 615 was expressed and the secreted protein purified from conditioned medium. The molecular masses of recombinant mouse MEP1A are similar to those observed for the alpha  subunit of rat Meprin A (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.