CDC73/HRPT2 Antibody

Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184] - Detection of human CDC73/HRPT2 (Parafibromin) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC73/HRPT2 antibody NB200-184 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated CDC73/HRPT2, NB200-184 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CDC73/HRPT2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Parafibromin using the numbering given in TrEMBL entry Q6P1J9 (GeneID 79577).
• Specificity: NB200-184 is specific for human Parafibromin protein.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC73
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:5000-1:20000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
• Control:

  CDC73/HRPT2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC73/HRPT2 Antibody

• C1orf28hyperparathyroidism 2 (with jaw tumor)
• CDC73
• cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S.cerevisiae)
• Cell division cycle protein 73 homolog
• chromosome 1 open reading frame 28
• HPTJT
• HPT-JT
• HRPT2
• HRPT2FLJ23316
• Hyperparathyroidism 2 protein
• HYX
• Parafibromin

Background

Parafibromin, the product of gene HPRT2, acts as a tumor suppressor protein by inhibiting cell proliferation and blocking expression of cyclin D1 when overexpressed. Parafibromin has also been shown to bind with the PAF1 complex / RNA polymerase II which is involved with transcription elongation and the RNA processing pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDC73/HRPT2 Antibody (NB200-184)(11)

Publications using NB200-184

• Tathe P, Chowdary KVSR, Murmu KC et al. SHP-1 dephosphorylates histone H2B to facilitate its ubiquitination during transcription The EMBO journal Aug 8 2022 [PMID: 35938192]
• Wu J, Xu W. Histone H3R17me2a mark recruits human RNA polymerase-associated factor 1 complex to activate transcription. Proc Natl Acad Sci U S A 2012 Apr [PMID: 22451921]
• Ruepp MD, Vivarelli S, Pillai RS et al. The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3'-end processing of animal histone mRNAs. Nucleic Acids Res 2010 Nov [PMID: 20634199]
• Yang YJ, Han JW, Youn HD et al. The tumor suppressor, parafibromin, mediates histone H3 K9 methylation for cyclin D1 repression. Nucleic Acids Res 2010 Jan [PMID: 19906718]
• Rozenblatt-Rosen O, Nagaike T, Francis JM et al. The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. Proc Natl Acad Sci U S A 2009 Jan [PMID: 19136632]
• Lin L, Zhang JH, Panicker LM et al. The parafibromin tumor suppressor protein inhibits cell proliferation by repression of the c-myc proto-oncogene. Proc Natl Acad Sci U S A 2008 Nov [PMID: 18987311]
• Tran H, Hamada F, Schwarz-Romond T et al. Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains. Genes Dev 2008 Feb [PMID: 18281465]
• Wang P, Bowl MR, Bender S et al. Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. Mol Cell Biol 2008 May [PMID: 18212049]
• Juhlin CC, Villablanca A, Sandelin K et al. Parafibromin immunoreactivity: its use as an additional diagnostic marker for parathyroid tumor classification. Endocr Relat Cancer 2007 Jun [PMID: 17639063]
• Mosimann C, Hausmann G, Basler K et al. Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo. Cell 2006 Apr [PMID: 16630820]
• Yart A, Gstaiger M, Wirbelauer C et al. The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II. Mol Cell Biol. 2005 Jun;25(12):5052-60. [PMID: 15923622]

Bioinformatics

• Gene Symbol: CDC73
• Entrez:
  • 79577(Human)
• Uniprot:
  • Q6P1J9()