CDC73/HRPT2 Antibody (NB200-184)

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Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified ...read more
Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human Parafibromin on HeLa whole cell lysate using NB200-184. In B, Parafibromin was immunoprecipitated by using NB200-184 at 3 mcg/10mg with or without ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

CDC73/HRPT2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Parafibromin using the numbering given in TrEMBL entry Q6P1J9 (GeneID 79577).
Specificity
NB200-184 is specific for human Parafibromin protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC73
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Positive Control
CDC73/HRPT2 Lysate (NBL1-09015)
Publications
Read Publications using NB200-184.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC73/HRPT2 Antibody

  • C1orf28hyperparathyroidism 2 (with jaw tumor)
  • CDC73
  • cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S.cerevisiae)
  • Cell division cycle protein 73 homolog
  • chromosome 1 open reading frame 28
  • HPTJT
  • HPT-JT
  • HRPT2
  • HRPT2FLJ23316
  • Hyperparathyroidism 2 protein
  • HYX
  • Parafibromin

Background

Parafibromin, the product of gene HPRT2, acts as a tumor suppressor protein by inhibiting cell proliferation and blocking expression of cyclin D1 when overexpressed. Parafibromin has also been shown to bind with the PAF1 complex / RNA polymerase II which is involved with transcription elongation and the RNA processing pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for CDC73/HRPT2 Antibody (NB200-184)(10)


Showing Publications 1 - 10 of 10.
Publications using NB200-184 Applications Species
Wu J, Xu W. Histone H3R17me2a mark recruits human RNA polymerase-associated factor 1 complex to activate transcription. Proc Natl Acad Sci U S A 2012 Apr [PMID: 22451921]
Ruepp MD, Vivarelli S, Pillai RS et al. The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3'-end processing of animal histone mRNAs. Nucleic Acids Res 2010 Nov [PMID: 20634199]
Yang YJ, Han JW, Youn HD et al. The tumor suppressor, parafibromin, mediates histone H3 K9 methylation for cyclin D1 repression. Nucleic Acids Res 2010 Jan [PMID: 19906718]
Rozenblatt-Rosen O, Nagaike T, Francis JM et al. The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. Proc Natl Acad Sci U S A 2009 Jan [PMID: 19136632]
Lin L, Zhang JH, Panicker LM et al. The parafibromin tumor suppressor protein inhibits cell proliferation by repression of the c-myc proto-oncogene. Proc Natl Acad Sci U S A 2008 Nov [PMID: 18987311]
Tran H, Hamada F, Schwarz-Romond T et al. Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains. Genes Dev 2008 Feb [PMID: 18281465]
Wang P, Bowl MR, Bender S et al. Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. Mol Cell Biol 2008 May [PMID: 18212049]
Juhlin CC, Villablanca A, Sandelin K et al. Parafibromin immunoreactivity: its use as an additional diagnostic marker for parathyroid tumor classification. Endocr Relat Cancer 2007 Jun [PMID: 17639063]
Mosimann C, Hausmann G, Basler K et al. Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo. Cell 2006 Apr [PMID: 16630820]
Yart A, Gstaiger M, Wirbelauer C et al. The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II. Mol Cell Biol. 2005 Jun;25(12):5052-60. [PMID: 15923622]

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDC73
Entrez
Uniprot