MEF2C [p Ser222] Antibody - Azide Free

Western Blot: MEF2C [p Ser222] Antibody [NBP2-60774] - Western blot of OCIAML2 lysate showing specificimmunolabeling of the ~51 kDa MEF2C phosphorylated atSer222 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where the immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase,1200 units for 30 minutes).

• Reactivity: Hu, Mu, Rt, Bv, Ch, Gp, Pm, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

MEF2C [p Ser222] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser222 conjugated to KLH. Accession # Q06413
• Modification: p Ser222
• Specificity: Specific for endogenous levels of the ~51 kDa MEF2C protein phosphorylated at Ser222. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Mouse (100%), Rat (100%), Chicken (100%), Sheep (100%), Primate (100%), Guinea Pig (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MEF2C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for MEF2C [p Ser222] Antibody - Azide Free

• DEL5q14.3
• MEF2C
• myocyte enhancer factor 2C
• myocyte-specific enhancer factor 2C
• polypeptide C

Background

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture . Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEF2C