MEF2C Antibody

Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP2-17260] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MEF2C protein stained by MEF2C antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse brain. MEF2C stained by MEF2C antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse fore brain. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Saos2 xenograft. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Frozen: MEF2C Antibody [NBP2-17260] - Mouse cerebral cortex. Green: MEF2C stained by MEF2C antibody diluted at 1:250.Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Human breast carcinoma. MEF2C stained by MEF2C antibody diluted at 1:250.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: MEF2C Antibody [NBP2-17260] - K562 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of MEF2C protein by 4 ug MEF2C antibody 5 % SDS-PAGE The immunoprecipitated MEF2C protein was detected by MEF2C antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt, Po, Bv, RM, Sh, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MEF2C Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MEF2C. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Porcine (97%), Bovine (100%), Rhesus Macaque (100%), Sheep (97%), Xenopus (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MEF2C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: IHC-Fr Assay dependent.
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (90%). Use in Rat reported in scientific literature (PMID:33125406).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS,20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for MEF2C Antibody

• DEL5q14.3
• MEF2C
• myocyte enhancer factor 2C
• myocyte-specific enhancer factor 2C
• polypeptide C

Background

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MEF2C Antibody (NBP2-17260)(2)

Publications using NBP2-17260

• Bowles Kr, Silva Mc, Whitney K Et Al. ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids Cell 2021-07-21 [PMID: 34314701] (IF/IHC)
• Ogura Y, Kakehashi C, Yoshihara T et al Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats PLoS One 2020-10-30 [PMID: 33125406] (WB, Rat)
  
  Details:
  Citation using the HRP version of this antibody.

Bioinformatics

• Gene Symbol: MEF2C