MEF2C Antibody

Images

 
Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP2-17260] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MEF2C protein stained by MEF2C antibody diluted at 1:1000. Red: phalloidin, a ...read more
Immunohistochemistry-Frozen: MEF2C Antibody [NBP2-17260] - Mouse cerebral cortex. Green: MEF2C stained by MEF2C antibody diluted at 1:250.Blue: Fluoroshield with DAPI.
Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more
Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse fore brain. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Saos2 xenograft. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: MEF2C Antibody [NBP2-17260] - K562 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of MEF2C protein by 4 ug MEF2C antibody 5 % SDS-PAGE The immunoprecipitated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RM, Sh, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MEF2C Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MEF2C. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Porcine (97%), Bovine (100%), Rhesus Macaque (100%), Sheep (97%), Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MEF2C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:5000-1:20000
Application Notes
IHC-Fr Assay dependent.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-17260 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Xenopus laevis (90%). Use in Rat reported in scientific literature (PMID:33125406).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS,20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for MEF2C Antibody

  • DEL5q14.3
  • MEF2C
  • myocyte enhancer factor 2C
  • myocyte-specific enhancer factor 2C
  • polypeptide C

Background

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MEF2C Antibody (NBP2-17260)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MEF2C