MEF2C Antibody (CL0368) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.

Staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.

Staining of human duodenum shows strong nuclear immunoreactivity in a subset of lymphoid cells.

Staining of human skeletal muscle shows moderate nuclear positivity in muscle fibers.

Staining of human pancreas shows absence of staining (negative control).

Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody) .

Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MEF2C antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human tonsil shows strong nuclear immunoreactivity in a subset of lymphoid cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0368
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MEF2C Antibody (CL0368) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q06413, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MEF2C
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF,Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for MEF2C Antibody (CL0368) - Azide and BSA Free

• DEL5q14.3
• MEF2C
• myocyte enhancer factor 2C
• myocyte-specific enhancer factor 2C
• polypeptide C

Background

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture . Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEF2C