MEF2C Antibody (CL0368) - BSA Free

Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MEF2C antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human tonsil shows strong nuclear immunoreactivity in a subset of lymphoid cells.

Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human duodenum shows strong nuclear immunoreactivity in a subset of lymphoid cells.

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human pancreas shows absense of staining (negative control).

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human skeletal muscle shows moderate nuclear positivity in muscle fibers.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, KD
• Clone: CL0368
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

MEF2C Antibody (CL0368) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q06413, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MEF2C
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  MEF2C Recombinant Protein Antigen (NBP2-52925PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for MEF2C Antibody (CL0368) - BSA Free

• DEL5q14.3
• MEF2C
• myocyte enhancer factor 2C
• myocyte-specific enhancer factor 2C
• polypeptide C

Background

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture . Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEF2C