MED17 Antibody (1B5)

Images

 
Western Blot: MED17 Antibody (1B5) [H00009440-M02] - CRSP6 monoclonal antibody (M02), clone 1B5 Analysis of CRSP6 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: MED17 Antibody (1B5) [H00009440-M02] - Analysis of monoclonal antibody to CRSP6 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: MED17 Antibody (1B5) [H00009440-M02] - Detection limit for recombinant GST tagged CRSP6 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MED17 Antibody (1B5) Summary

Immunogen
CRSP6 (AAH21101, 551 a.a. ~ 652 a.a) partial recombinant protein with GST tag.FSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL
Specificity
CRSP6 (1B5)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MED17
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Reviewed Applications
Read 1 Review rated 4
using
H00009440-M02 in the following applications:

Publications
Read Publication using H00009440-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED17 Antibody (1B5)

  • Activator-recruited cofactor 77 kDa component
  • Cofactor required for Sp1 transcriptional activation subunit 6
  • cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa
  • CRSP6FLJ10812
  • CRSP77cofactor required for Sp1 transcriptional activation, subunit 6 (77kD)
  • DRIP77
  • DRIP80ARC77
  • mediator complex subunit 17Vitamin D3 receptor-interacting protein complex 80 kDa component
  • mediator of RNA polymerase II transcription subunit 17
  • Thyroid hormone receptor-associated protein complex 80 kDa component
  • Transcriptional coactivator CRSP77
  • Trap80
  • TRAP80CRSP complex subunit 6

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MED17 Antibody (H00009440-M02)(1)

We have publications tested in 1 confirmed species: Human.


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Review for MED17 Antibody (H00009440-M02) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00009440-M02:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot MED17 H00009440-M02
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reviewed by:
Anonymous
WB Human 05/26/2016
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Summary

ApplicationWestern Blot
Sample TestedA549 nuclear lysate
SpeciesHuman
Lot7166-1b5-00Ka6

Blocking

Blocking Details5% milk in TBST

Details

Detection NotesSpecific bands seen about 80kD and 120 kD

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MED17 Antibody (H00009440-M02) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
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2
0
1
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Anonymous
05/26/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol MED17
Entrez
OMIM
Uniprot