MED17 Antibody (1B5) - Azide and BSA Free

Western Blot: MED17 Antibody (1B5) [H00009440-M02] - CRSP6 monoclonal antibody (M02), clone 1B5 Analysis of CRSP6 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: MED17 Antibody (1B5) [H00009440-M02] - Analysis of monoclonal antibody to CRSP6 on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: MED17 Antibody (1B5) [H00009440-M02] - Detection limit for recombinant GST tagged CRSP6 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MED17 Antibody (1B5) - Azide and BSA Free Summary

• Immunogen: CRSP6 (AAH21101, 551 a.a. ~ 651 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL
• Specificity: CRSP6 (1B5)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MED17
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED17 Antibody (1B5) - Azide and BSA Free

• Activator-recruited cofactor 77 kDa component
• Cofactor required for Sp1 transcriptional activation subunit 6
• cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa
• CRSP6FLJ10812
• CRSP77cofactor required for Sp1 transcriptional activation, subunit 6 (77kD)
• DRIP77
• DRIP80ARC77
• mediator complex subunit 17Vitamin D3 receptor-interacting protein complex 80 kDa component
• mediator of RNA polymerase II transcription subunit 17
• Thyroid hormone receptor-associated protein complex 80 kDa component
• Transcriptional coactivator CRSP77
• Trap80
• TRAP80CRSP complex subunit 6

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED17 Antibody (H00009440-M02)(1)

Publication using H00009440-M02

• Tsutsui T, Fukasawa R, Shinmyouzu K et al. Mediator Complex Recruits Epigenetic Regulators via Its Two Cyclin-dependent Kinase Subunits to Repress Transcription of Immune Response Genes. J Biol Chem 2013-07-19 [PMID: 23749998] (Human)

Review for MED17 Antibody (H00009440-M02) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 05/26/2016

Summary

• Application: Western Blot
• Sample Tested: A549 nuclear lysate
• Species: Human
• Lot: 7166-1b5-00Ka6

Bioinformatics

• Gene Symbol: MED17
• Entrez:
  • 9440(Human)
• OMIM:
  • 603810(Human)
• Uniprot:
  • AAH21101(Human)