MECR Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MECR Antibody [NBP1-85657] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.

Staining of human cerebral cortex, placenta, skeletal muscle and testis using Anti-MECR antibody NBP1-85657 (A) shows similar protein distribution across tissues to independent antibody NBP1-85659 (B).

Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Analysis using Anti-MECR antibody NBP1-85657 (A) shows similar pattern to independent antibody NBP1-85659 (B).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MECR Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MECR Antibody - BSA Free (NBP1-85657) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SSTELLRQLARGGTMVTYGGMAKQPVVASVSLLIFKDLKLRGFWLSQWKKDHSPDQFKELILTLCDLIRRGQLTAPACSQVPLQDYQSALEASMKPFISSKQILTM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MECR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MECR Protein (NBP1-85657PEP)

Reactivity Notes

Rat (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MECR Antibody - BSA Free

• CGI-63
• EC 1.3.1.38
• FASN2B
• homolog of yeast 2-enoyl thioester reductase
• HsNrbf-1
• mitochondrial 2-enoyl thioester reductase
• mitochondrial trans-2-enoyl-CoA reductase
• NBRF1
• NRBF-1
• NRBF1hsNrbf-1
• nuclear receptor binding factor 1
• Nuclear receptor-binding factor 1
• trans-2-enoyl-CoA reductase, mitochondrial

Background

MECR catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- ordeoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue ofpyrimidine bases for nucleotide synthesi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MECR