MDMX Antibody (2D10F4)

Western Blot: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of Hela (left) and L-02 (right) cells using MDM4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of human Tonsil tissues using MDM4 mouse mAb

Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 mouse mAb.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2D10F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MDMX Antibody (2D10F4) Summary

• Immunogen: Purified recombinant fragment of human MDMX expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MDM4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MDMX Antibody (2D10F4)

• DKFZp781B1423
• double minute 4 homolog
• Double minute 4 protein
• double minute 4, human homolog of; p53-binding protein
• HDMX
• Mdm2-like p53-binding protein
• Mdm4 p53 binding protein homolog (mouse)
• MDM4 Regulator of P53
• MDM4
• Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
• Mdm4, transformed 3T3 cell double minute 4, p53 binding protein
• MDM4-related protein 1
• MDMX
• MDMXMGC132766
• mouse double minute 4, human homolog of; p53-binding protein
• MRP1
• p53-binding protein Mdm4
• protein Mdm4
• Protein Mdmx

Background

Mdm4 p53 binding protein homolog (mouse). The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDMX Antibody (NBP1-28862)(2)

Publications using NBP1-28862

• Woodfield SE, Shi Y, Patel RH et al. MDM4 inhibition: a novel therapeutic strategy to reactivate p53 in hepatoblastoma Scientific reports 2021-02-03 [PMID: 33536467] (WB, Human)
• Karki A, Putra J, Kim SS et al. MDM4 expression in fibrolamellar hepatocellular carcinoma Oncol. Rep. 2019-07-18 [PMID: 31322272] (WB, IF/IHC, Human)

Bioinformatics

• Gene Symbol: MDM4
• Entrez:
  • 4194(Human)
• Uniprot:
  • O15151(Human)