MDH1 Antibody (2B11-B7) - Azide and BSA Free

Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - Analysis of MDH1 expression in transfected 293T cell line by MDH1 monoclonal antibody (M01), clone 2B11-B7.Lane 1: MDH1 transfected lysate(36.4 KDa).Lane 2: Non-transfected lysate.

Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - MDH1 monoclonal antibody (M01), clone 2B11-B7. Analysis of MDH1 expression in HeLa.

Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - MDH1 monoclonal antibody (M01), clone 2B11-B7. Analysis of MDH1 expression in HepG2.

Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - MDH1 monoclonal antibody (M01), clone 2B11-B7. Analysis of MDH1 expression in HL-60.

Sandwich ELISA: MDH1 Antibody (2B11-B7) [H00004190-M01] - Detection limit for recombinant GST tagged MDH1 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2B11-B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MDH1 Antibody (2B11-B7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MDH1 (AAH01484.1, 1 a.a. ~ 334 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA
• Localization: Cytoplasmic and Mitochondrial
• Specificity: MDH1 - malate dehydrogenase 1, NAD (soluble)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MDH1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MDH1 Antibody (2B11-B7) - Azide and BSA Free

• aromatic alpha-keto acid reductase
• cytoplasmic
• Cytosolic malate dehydrogenase
• DEE88
• Diiodophenylpyruvate reductase
• EC 1.1.1.37
• EC 1.1.1.96
• EIEE88
• epididymis secretory protein Li 32
• HEL-S-32
• KAR
• malate dehydrogenase 1, NAD (soluble)
• Malate dehydrogenase, cytoplasmic
• malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal
• MDH1
• MDHA
• MDH-s
• MGC:1375
• MOR2
• soluble malate dehydrogenase

Background

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MDH1
• Entrez:
  • 4190(Human)
• OMIM:
  • 154200(Human)
• Uniprot:
  • AAH01484(Human)