| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89515-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22162-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08427 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33708-25ul | |||
![Immunocytochemistry/Immunofluorescence: MDH1 Antibody [NBP1-89515] - Staining of human cell line U-251 MG shows localization to cytosol & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MDH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89515-img0007.jpg "Immunocytochemistry/Immunofluorescence: MDH1 Antibody [NBP1-89515] - Staining of human cell line U-251 MG shows localization to cytosol & centrosome. Antibody staining is shown in green.")
![Western Blot: MDH1 Antibody (JE35-03) [NBP3-32582] - Western blot analysis of MDH1 on different lysates with Rabbit anti-MDH1 antibody (NBP3-32582) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: U-937 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: NIH/3T3 cell lysate (20 ug/Lane) Lane 6: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 6 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32582) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32582_rabbit-mdh1-mab-je35-03-278202416331296.jpg)
![Immunocytochemistry/ Immunofluorescence: MDH1 Antibody (JE35-03) [NBP3-32582] - Immunocytochemistry analysis of U-937 cells labeling MDH1 with Rabbit anti-MDH1 antibody (NBP3-32582) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MDH1 antibody (NBP3-32582) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32582_rabbit-mdh1-mab-je35-03-2920241114837.jpg)
![Flow Cytometry: MDH1 Antibody (JE35-03) [NBP3-32582] - Flow cytometric analysis of U-937 cells labeling MDH1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32582, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32582_rabbit-mdh1-mab-je35-03-392024884643.jpg)
![Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - Analysis of MDH1 expression in transfected 293T cell line by MDH1 monoclonal antibody (M01), clone 2B11-B7.Lane 1: MDH1 transfected lysate(36.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MDH1-Antibody-2B11-B7-Western-Blot-H00004190-M01-img0008.jpg "Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - Analysis of MDH1 expression in transfected 293T cell line by MDH1 monoclonal antibody (M01), clone 2B11-B7.Lane 1: MDH1 transfected lysate(36.4 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - MDH1 monoclonal antibody (M01), clone 2B11-B7. Analysis of MDH1 expression in HeLa.](http://images.novusbio.com/fullsize/MDH1-Antibody-2B11-B7-Western-Blot-H00004190-M01-img0005.jpg)
![Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - MDH1 monoclonal antibody (M01), clone 2B11-B7. Analysis of MDH1 expression in HepG2.](http://images.novusbio.com/fullsize/MDH1-Antibody-2B11-B7-Western-Blot-H00004190-M01-img0006.jpg)
![Western Blot: MDH1 Antibody (2B11-B7) [H00004190-M01] - MDH1 monoclonal antibody (M01), clone 2B11-B7. Analysis of MDH1 expression in HL-60.](http://images.novusbio.com/fullsize/MDH1-Antibody-2B11-B7-Western-Blot-H00004190-M01-img0007.jpg)
![Sandwich ELISA: MDH1 Antibody (2B11-B7) [H00004190-M01] - Detection limit for recombinant GST tagged MDH1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDH1-Antibody-2B11-B7-Sandwich-ELISA-H00004190-M01-img0004.jpg)
![Western Blot: MDH1 Antibody (SR1599) [NBP3-22162] - Western blot analysis using NBP3-22162 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22162_rabbit-mdh1-mab-sr1599-5720231658217.jpg "Western Blot: MDH1 Antibody (SR1599) [NBP3-22162] - Western blot analysis using NBP3-22162 in HeLa cell lysate.")
![Western Blot: MDH1 Antibody [NBP1-77780] - Malate Dehydrogenase Antibody](http://images.novusbio.com/fullsize/MDH1-Antibody-Western-Blot-NBP1-77780-img0001.jpg "Western Blot: MDH1 Antibody [NBP1-77780] - Malate Dehydrogenase Antibody")
![Western Blot: MDH1 Antibody (8U9X1) [NBP3-16860] - Western blot analysis of extracts of various cell lines, using MDH1 Rabbit mAb (NBP3-16860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MDH1-Antibody-8U9X1-Western-Blot-NBP3-16860-img0001.jpg "Western Blot: MDH1 Antibody (8U9X1) [NBP3-16860] - Western blot analysis of extracts of various cell lines, using MDH1 Rabbit mAb (NBP3-16860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: MDH1 Antibody (CPTC-MDH1-1) [NBP3-07289] - Western Blot Analysis of HeLa, HepG2, Jurkat, HEP2, U937, PC3 cell lysates using MDH1 Mouse Monoclonal Antibody (CPTC-MDH1-1).](http://images.novusbio.com/fullsize/MDH1-Antibody-CPTC-MDH1-1-Western-Blot-NBP3-07289-img0001.jpg "Western Blot: MDH1 Antibody (CPTC-MDH1-1) [NBP3-07289] - Western Blot Analysis of HeLa, HepG2, Jurkat, HEP2, U937, PC3 cell lysates using MDH1 Mouse Monoclonal Antibody (CPTC-MDH1-1).")
![Immunohistochemistry-Paraffin: MDH1 Antibody [NBP2-33708] - Staining of human stomach, lower shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MDH1-Antibody-Immunohistochemistry-Paraffin-NBP2-33708-img0001.jpg "Immunohistochemistry-Paraffin: MDH1 Antibody [NBP2-33708] - Staining of human stomach, lower shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.")
![Western Blot: MDH1 Antibody [NBP1-54797] - Jurkat cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/MDH1-Antibody-Western-Blot-NBP1-54797-img0002.jpg "Western Blot: MDH1 Antibody [NBP1-54797] - Jurkat cell lysate, concentration 2.5 ug/ml.")
![SDS-Page: Recombinant Human MDH1 His Protein [NBP1-45315] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human MDH1 His Protein-SDS-Page-NBP1-45315-img0003.jpg "SDS-Page: Recombinant Human MDH1 His Protein [NBP1-45315] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: Recombinant E. coli MDH1 Protein [NBP1-99038] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-E.-coli-MDH1-Protein-SDS-Page-NBP1-99038-img0001.jpg "SDS-Page: Recombinant E. coli MDH1 Protein [NBP1-99038] - 15% SDS-PAGE (3ug)")
![Western Blot: MDH1 Overexpression Lysate (Adult Normal) [NBL1-12964] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MDH1.](http://images.novusbio.com/fullsize/MDH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12964-img0002.jpg "Western Blot: MDH1 Overexpression Lysate (Adult Normal) [NBL1-12964] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MDH1.")