MDA5 Antibody - BSA Free

Western Blot: MDA5 Antibody [NBP1-76760] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.

Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76760] - Human lymph node tissue with MDA5 antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76760] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.

Immunohistochemistry: MDA5 Antibody - BSA Free [NBP1-76760] - Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MDA5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MDA5 Antibody - BSA Free (NBP1-76760) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-MDA5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5. The immunogen is located within the last 50 amino acids of MDA5. Amino Acid Squence: FKNNSTKKQYKKWVEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFIH1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-4 ug/ml
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  MDA5 Peptide (NBP1-76760PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MDA5 Antibody - BSA Free

• AGS7
• CADM-140 autoantigen
• Clinically amyopathic dermatomyositis autoantigen 140 kDa
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• EC 3.6.4.13
• Helicard
• Helicase with 2 CARD domains
• Hlcd
• IDDM19
• IFIH1
• IMD95
• interferon induced with helicase C domain 1
• interferon-induced helicase C domain-containing protein 1
• Interferon-induced with helicase C domain protein 1
• MDA5
• MDA-5
• MDA-5MGC133047
• MDA5RNA helicase-DEAD box protein 116
• melanoma differentiation associated protein-5
• Melanoma differentiation-associated protein 5
• Murabutide down-regulated protein
• RH116
• SGMRT1

Background

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kappaB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDA5 Antibody (NBP1-76760)(1)

Publication using NBP1-76760

• Cali M. McEntee, Alyssa N. Cavalier, Thomas J. LaRocca ADAR1 suppression causes interferon signaling and transposable element transcript accumulation in human astrocytes Frontiers in Molecular Neuroscience 2023-10-25 [PMID: 38035265]

Bioinformatics

• Gene Symbol: IFIH1
• Uniprot:
  • NP_071451(Human)