MDA5 Antibody (SR1076) - BSA Free

Western blot shows lysates of DMS 53 parental cell line and MDA5 knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with MDA5 Antibody (SR1076) (Catalog # NBP3-21840) followed by HRP-conjugated secondary antibody. A specific band was detected for MDA5 at approximately 116.6 kDa (as indicated) in the parental DMS 53 cell line, but is not detectable in knockdown DMS 53 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] - Western blot analysis using NBP3-21840 in THP1 cell lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF, Mycoplasma
• Clone: SR1076
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MDA5 Antibody (SR1076) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human MDA5 (Uniprot #: Q9BYX4)
• Specificity: Detects endogenous levels of total MDA5
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFIH1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Knockdown Validated Knockdown Validated from YCharOS Inc. (YCharOS.com)
  • Western Blot 1:500-1:2000
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MDA5 Antibody (SR1076) - BSA Free

• AGS7
• CADM-140 autoantigen
• Clinically amyopathic dermatomyositis autoantigen 140 kDa
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• EC 3.6.4.13
• Helicard
• Helicase with 2 CARD domains
• Hlcd
• IDDM19
• IFIH1
• IMD95
• interferon induced with helicase C domain 1
• interferon-induced helicase C domain-containing protein 1
• Interferon-induced with helicase C domain protein 1
• MDA5
• MDA-5
• MDA-5MGC133047
• MDA5RNA helicase-DEAD box protein 116
• melanoma differentiation associated protein-5
• Melanoma differentiation-associated protein 5
• Murabutide down-regulated protein
• RH116
• SGMRT1

Background

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFIH1