MDA5 Antibody (3D12) - BSA Free

Western Blot: MDA5 Antibody (3D12) [NBP3-15166] - Positive WB detected in: THP-1 whole cell lysate, Mouse lung tissue. All lanes: MDA5 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 117, 26 KDa. Observed band size: 117 KDa.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 3D12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MDA5 Antibody (3D12) - BSA Free Summary

• Description: Novus Biologicals Rabbit MDA5 Antibody (3D12) - BSA Free (NBP3-15166) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human MDA5. UniProt ID (Q9BYX4).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFIH1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MDA5 Antibody (3D12) - BSA Free

• AGS7
• CADM-140 autoantigen
• Clinically amyopathic dermatomyositis autoantigen 140 kDa
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• EC 3.6.4.13
• Helicard
• Helicase with 2 CARD domains
• Hlcd
• IDDM19
• IFIH1
• IMD95
• interferon induced with helicase C domain 1
• interferon-induced helicase C domain-containing protein 1
• Interferon-induced with helicase C domain protein 1
• MDA5
• MDA-5
• MDA-5MGC133047
• MDA5RNA helicase-DEAD box protein 116
• melanoma differentiation associated protein-5
• Melanoma differentiation-associated protein 5
• Murabutide down-regulated protein
• RH116
• SGMRT1

Background

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFIH1