MDA5 Antibody (2N8Z5)

Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of BxPC-3 cells, using MDA5 antibody (NBP3-16132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 2N8Z5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MDA5 Antibody (2N8Z5) Summary

• Description: Novus Biologicals Rabbit MDA5 Antibody (2N8Z5) (NBP3-16132) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human MDA5 (Q9BYX4). PSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQY
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFIH1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MDA5 Antibody (2N8Z5)

• AGS7
• CADM-140 autoantigen
• Clinically amyopathic dermatomyositis autoantigen 140 kDa
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• EC 3.6.4.13
• Helicard
• Helicase with 2 CARD domains
• Hlcd
• IDDM19
• IFIH1
• IMD95
• interferon induced with helicase C domain 1
• interferon-induced helicase C domain-containing protein 1
• Interferon-induced with helicase C domain protein 1
• MDA5
• MDA-5
• MDA-5MGC133047
• MDA5RNA helicase-DEAD box protein 116
• melanoma differentiation associated protein-5
• Melanoma differentiation-associated protein 5
• Murabutide down-regulated protein
• RH116
• SGMRT1

Background

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFIH1