MCT1/SLC16A1 Antibody (9D12) - BSA Free

Western Blot: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. All lanes: MCT1/SLC16A1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 47 KDa. Observed band size: 54 KDa.

Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - IHC image of MCT1/SLC16A1 antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - IHC image of MCT1/SLC16A1 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 9D12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MCT1/SLC16A1 Antibody (9D12) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human MCT1/SLC16A1. UniProt ID (P53985).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC16A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MCT1/SLC16A1 Antibody (9D12) - BSA Free

• FLJ36745
• HHF7
• MCT 1
• MCT
• MCT1
• MCT1MGC44475
• member 1
• monocarboxylate transporter 1
• SLC16A1
• Solute carrier family 16 member 1
• solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

Background

The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC16A1