MCPIP1/ZC3H12A Antibody (604421) [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 604421
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

MCPIP1/ZC3H12A Antibody (604421) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human MCPIP1
  Asp426-Glu599
  Accession # Q5D1E8
• Specificity: Detects human MCPIP1 in direct ELISAs and human and mouse MCPIP1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human MCPIP3 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCPIP1/ZC3H12A Antibody (604421) [Alexa Fluor® 700]

• MCPIP1
• Reg1
• Regnase-1
• ZC3H12A
• zinc finger CCCH-type containing 12A

Background

Human MCP-induced protein (MCPIP), also known as ZC3H12A, is an approximately 66 kDa intracellular protein that contains one RNAse domain (aa 134-290), one zinc finger (aa 301-324), and a proline-rich region (aa 458-536). Within aa 426-599, human MCPIP shares approximately 79% aa sequence identity with mouse and rat MCPIP. Its expression is induced by inflammatory stimulation and cellular stress. It acts to dampen inflammatory responses by promoting the degradation of proinflammatory cytokine mRNAs, inhibiting NFkB activation, and antagonizing TLR signaling. MCPIP exhibits deubiquitinase activity and inhibits the biogenesis of miRNA. It also enhances inflammation-induced angiogenesis, osteocyte and adipocyte differentiation, and the cell death of cardiac myocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.