MCM7 Antibody

Images

 
Western Blot: MCM7 Antibody [NBP1-31350] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.
Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was ...read more
Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MCM7 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MCM7. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (92%), Rat (93%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Chromatin Immunoprecipitation (ChIP)
Publications
Read Publications using
NBP1-31350 in the following applications:

  • WB
    2 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for MCM7 Antibody

  • CDC47 homolog
  • CDC47
  • CDC47P85MCM
  • DNA replication licensing factor MCM7
  • EC 3.6.4.12
  • homolog of S. cerevisiae Cdc47
  • MCM2
  • MCM2PNAS146
  • MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
  • MCM7
  • minichromosome maintenance complex component 7
  • minichromosome maintenance deficient (S. cerevisiae) 7
  • minichromosome maintenance deficient 7
  • P1.1-MCM3
  • P1CDC47
  • P85MCM
  • PNAS146
  • PPP1R104
  • Protein Phosphatase 1

Background

The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MCM7 Antibody (NBP1-31350)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MCM7