![Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31350-img0004.jpg "Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit MCM7 Antibody - BSA Free (NBP1-31350) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-MCM7 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MCM7. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Mouse (92%), Rat (93%), Bovine (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MCM7 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 81 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Yongkang Yang |
WB | Human | 11/28/2024 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for MCM7 Antibody (NBP1-31350)Find related products by research area.
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| Gene Symbol | MCM7 |
![Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunohistochemistry-Paraffin-NBP1-31350-img0005.jpg "Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.")
![Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/MCM7-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31350-img0008.jpg "Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.")
![Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 antibody [N2C2], Internal. 7.5 % SDS-PAGE. The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunoprecipitation-NBP1-31350-img0007.jpg "Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 antibody [N2C2], Internal. 7.5 % SDS-PAGE. The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Western Blot: Rabbit Polyclonal MCM7 Antibody [NBP1-31350] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. MCM7 Antibody (NBP1-31350) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-212202416030..jpg "Western Blot: Rabbit Polyclonal MCM7 Antibody [NBP1-31350] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. MCM7 Antibody (NBP1-31350) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.")
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518243316.jpg "Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-141120251843245.jpg "Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518381712.jpg "Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518273411.jpg "Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
