MCM4 Antibody - Azide and BSA Free

Western Blot: MCM4 Antibody [H00004173-B01P] - Analysis of MCM4 expression in human placenta.

Immunocytochemistry/Immunofluorescence: MCM4 Antibody [H00004173-B01P] - Analysis of purified antibody to MCM4 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: MCM4 Antibody [H00004173-B01P] - Analysis of purified antibody to MCM4 on formalin-fixed paraffin-embedded human tonsil. (antibody concentration 3 ug/ml)

Western Blot: MCM4 Antibody [H00004173-B01P] - Analysis of MCM4 expression in HeLa.

Western Blot: MCM4 Antibody [H00004173-B01P] - Analysis of MCM4 expression in transfected 293T cell line by MCM4 polyclonal antibody. Lane 1: MCM4 transfected lysate(94.93 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MCM4 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MCM4 (AAH31061.1, 1 a.a. - 863 a.a.) full-length human protein. MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKTVRLL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MCM4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: It has been used for WB and IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCM4 Antibody - Azide and BSA Free

• CDC21 homolog
• CDC21
• CDC54
• DNA replication licensing factor MCM4
• EC 3.6.4.12
• hCdc21
• homolog of S. pombe cell devision cycle 21
• MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)
• MCM4 minichromosome maintenance deficient 4
• MGC33310
• minichromosome maintenance complex component 4
• minichromosome maintenance deficient 4
• P1-Cdc21

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM4 Antibody (H00004173-B01P)(2)

Publications using H00004173-B01P

• Polasek-Sedlackova H, Miller TCR, Krejci J et al. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes Nature communications 2022-10-14 [PMID: 36241664] (ICC/IF, Human)
• Gallo D, Young JTF, Fourtounis J et al. CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition Nature 2022-04-01 [PMID: 35444283] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: MCM4
• Uniprot:
  • AAH31061.1(Human)