Mcl-1 Antibody (HL1544) - Azide and BSA Free

Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded cat tissue. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissue. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ca, Fe
• Applications: WB, IHC
• Clone: HL1544
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Mcl-1 Antibody (HL1544) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Mcl-1 Antibody (HL1544) - Azide and BSA Free (NBP3-25560) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Mcl-1. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MCL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Mcl-1 Antibody (HL1544) - Azide and BSA Free

• BCL2L3
• bcl2-L-3
• BCL2L3MGC104264
• Bcl-2-like protein 3
• Bcl-2-related protein EAT/mcl1
• EAT
• induced myeloid leukemia cell differentiation protein Mcl-1
• Mcl1
• Mcl-1
• mcl1/EAT
• MCL1-ES
• MCL1L
• MCL1S
• MGC1839
• myeloid cell leukemia ES
• myeloid cell leukemia sequence 1 (BCL2-related)
• TM

Background

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCL1