MCAM/CD146 Antibody (MUC18/1130)

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Tonsil stained with MUC18 Monoclonal Antibody (MUC18/1130)

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - CD146 was stained in both human brain and pancreatic tissues. CD146 expression was high in pancreatic tissue, yet low in brain tissue as shown by the brown coloration. Image from a verified customer review.

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - CD146 Staining of Normal Human Tissues. Image from a verified customer review.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, ICC/IF
• Clone: MUC18/1130
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MCAM/CD146 Antibody (MUC18/1130) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47777)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human MCAM/CD146 protein (Uniprot: P43121)
• Localization: Cell surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCAM
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for MCAM/CD146 Antibody (MUC18/1130)

• CD146 antigen
• CD146
• cell surface glycoprotein MUC18
• Cell surface glycoprotein P1H12
• L-Gicerin
• MCAM
• melanoma adhesion molecule
• melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
• Melanoma-associated antigen A32
• Melanoma-associated antigen MUC18
• MUC18
• MUC18Gicerin

Background

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCAM/CD146 Antibody (NBP2-44512)(3)

Publications using NBP2-44512

• Liu Y, Zhou Y. Circ_0087960 stabilizes KDM5B by reducing SKP2 mediated ubiquitination degradation and promotes osteogenic differentiation in periodontal ligament stem cells Regen Ther 2022-03-01 [PMID: 35229010] (FLOW, Human)
  
  Details:
  Citation using the FITC version of this antibody.
• Sun H, England CG, Hernandez R et al. ImmunoPET for assessing the differential uptake of a CD146-specific monoclonal antibody in lung cancer Eur. J. Nucl. Med. Mol. Imaging 2016-06-25 [PMID: 27342417]
• Elahi FM, Harvey D, Altendahl M, Casaletto KB Endothelial-derived exosomes demonstrate a link between endothelial innate inflammation and brain dysfunction and injury in aging bioRxiv 2019 Jun 21 (IP, Human) Endothelial-derived exosomes demonstrate a link between endothelial innate inflammation and brain dysfunction and injury in aging. BioRxiv 2019-06-21 (IP, Human)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

Reviews for MCAM/CD146 Antibody (NBP2-44512) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, uman tissue.

reviewed by: Christopher England IHC-P uman tissue 12/05/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: brain and pancreatic tissue
• Species: uman tissue
• Lot: 4162-4P150217

Comments

• Comments: The antibody was used at a ratio of 1:200.

reviewed by: Christopher England IHC-P Human 06/04/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Normal Tissue Microarray
• Species: Human
• Lot: 4162-4P150217

Bioinformatics

• Gene Symbol: MCAM
• Uniprot:
  • P43121(Human)