MCAM/CD146 Antibody (c264)

Flow Cytometry: MCAM/CD146 Antibody (c264) [NBP1-28331] - Chicken thymocytes were stained with Mouse Anti-Chicken MCAM-UNLB (NBP1-28331) followed by Goat Anti-Mouse IgG2b.

Product Discontinued

MCAM/CD146 Antibody (c264) Summary

• Immunogen: Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos
• Specificity: Chicken MCAM, Mr 98 kDa (protein core approx. 84 kDa)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCAM
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells
• Application Notes: For flow cytometry, the suggested use of these reagents is in a final volume of 100 mL

Reactivity Notes

Chicken

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for MCAM/CD146 Antibody (c264)

• CD146 antigen
• CD146
• cell surface glycoprotein MUC18
• Cell surface glycoprotein P1H12
• L-Gicerin
• MCAM
• melanoma adhesion molecule
• melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
• Melanoma-associated antigen A32
• Melanoma-associated antigen MUC18
• MUC18
• MUC18Gicerin

Background

Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm's kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCAM