MBP Antibody (HL1033) - Azide and BSA Free

Immunohistochemistry-Paraffin: MBP Antibody (HL1033) [NBP3-13687] - MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:100.Blue: Hoechst 33342 staining.

Myelin basic protein antibody [HL1033] detects Myelin basic protein protein by immunohistochemical analysis.Sample: Paraffin-embedded rat hippocampus.Myelin basic protein stained by Myelin basic protein antibody [HL1033] (NBP3-13687) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic protein antibody [HL1033] (NBP3-13687) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody [HL1033] (NBP3-13687) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL1033
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MBP Antibody (HL1033) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse MBP. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:5000-1:500000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for MBP Antibody (HL1033) - Azide and BSA Free

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBP