MBL Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

MBL Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MBL (R&D Systems, Catalog # 2307-MB)
  Glu21-Ile248
  Accession # AAH96182
• Specificity: Detects human MBL in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse (rm) MBL and rmMBL‑2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MBL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human MBL Antibody (Catalog # AF2307)
  ELISA Detection:Human MBL Biotinylated Antibody (Catalog # BAF2307)
  Standard:Recombinant Human MBL (Catalog # 2307-MB)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MBL Antibody [Biotin]

• COLEC1
• COLEC1collectin-1
• Collectin-1
• HSMBPC
• Mannan-binding protein
• mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• mannose-binding lectin (protein C) 2, soluble
• Mannose-binding lectin
• mannose-binding protein C
• MBL
• MBL2
• MBL2D
• MBLmannan-binding lectin
• MBP
• MBP1
• MBP1mannose-binding lectin 2, soluble (opsonic defect)
• MBP-C
• MGC116832
• MGC116833

Background

Human mannose/mannan-binding lectin (MBL; also MBP-C) is a 25 kDa member of the collectin family of pattern-recognition molecules (1-3). It is a secreted glycoprotein that is synthesized as a 248 amino acid (aa) precursor that contains a 20 aa signal sequence, a 21 aa cysteine-rich region (with three cysteines) a 58 aa collagen-like segment and a 111 aa C-type lectin domain that binds to neutral bacterial carbohydrates (3, 4). The molecule is O-glycosylated and contains multiple hydroxylated prolines and lysines (3, 5). Functionally, the molecule operates as a multimer/oligomer. The basic structural unit is a homotrimer. The homotrimer is created by the formation of interchain disulfide bonds among the cysteine-rich regions, plus a helical interaction of the collagen-like domains of each participating polypeptide (5). Mutations in the collagen region are known to interfere with proper trimer and subsequent oligomer formation (6). Once formed, the trimer, as a unit, oligomerizes with other trimers to form high molecular weight complexes. Although the exact nature of these complexes are unclear, it would appear that a three trimer complex (230 kDa) and a four trimer complex (305 kDa) constitute much of the circulating MBL (7). It is within the context of these oligomers that MBL performs its functions. After secretion by hepatocytes, oligomerized MBL will both associate with serine proteases (MASP-1, -2, and -3) and bind to bacterial carbohydrates. If the MBL complex is small, opsonization of bacteria occurs. If the complex is large, the MASPs are engaged and a complement attack complex is generated, destroying bound bacteria (3, 7, 8). Human MBL is 63%, 61% and 65% aa identical to mouse, porcine and bovine MBL, respectively.

1. Gadjeva, M. et al. (2004) Mol. Immunol. 41:113.
2. Kilpatrick, D.C. (2003) Biochem. Soc. Trans. 31:745.
3. Presanis, J.S. et al. (2003) Biochem. Soc. Trans. 31:748.
4. Sastry, K. et al. (1989) J. Exp. Med. 170:1175.
5. Jensen, P.H. et al. (2005) J. Biol. Chem. 280:11043.
6. Larsen, F. et al. (2004) J. Biol. Chem. 279:21302.
7. Teillet, F. et al. (2005) J. Immunol. 174:2870.
8. Terai, I. et al. (2003) Eur. J. Immunol. 33:2755.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBL Antibody (BAF2307)(3)

Publications using BAF2307

• Alexandra T. Matola, Angéla Fülöp, Bernadette Rojkovich, György Nagy, Gabriella Sármay, Mihály Józsi et al. Autoantibodies against complement factor B in rheumatoid arthritis Frontiers in Immunology 2/20/2023 [PMID: 36891314]
• David Jamieson Pharmacogenetic association of MBL2 and CD95 polymorphisms with grade 3 infection following adjuvant therapy for breast cancer with doxorubicin and cyclophosphamide Eur. J. Cancer, 2016-12-08;71(0):15-24. 2016-12-08 [PMID: 27940354] (ELISA Development (Detection), Human)
• Thye T, Niemann S, Walter K, Homolka S, Intemann CD, Chinbuah MA, Enimil A, Gyapong J, Osei I, Owusu-Dabo E, Rusch-Gerdes S, Horstmann RD, Ehlers S, Meyer CG Variant G57E of Mannose Binding Lectin Associated with Protection against Tuberculosis Caused by Mycobacterium africanum but not by M. tuberculosis. PLoS ONE, 2011-06-10;6(6):e20908. 2011-06-10 [PMID: 21695215] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: MBL2
• Uniprot:
  • AAH96182()