MBD4 Antibody [FITC]

• Reactivity: Hu, Mu(-)
• Applications: ICC/IF, IHC, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

MBD4 Antibody [FITC] Summary

• Immunogen: A synthetic peptide made to an internal portion of the human MBD4 protein (between residues 200-250) [UniProt O95243]
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MBD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MBD4 Antibody [FITC]

• EC 3.2.2.-
• G/T mismatch glycosylase
• G/U mismatch glycosylase
• MBD4
• MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics
• methyl-CpG binding domain protein 4
• methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase
• Methyl-CpG-binding endonuclease 1
• Methyl-CpG-binding protein MBD4
• Mismatch-specific DNA N-glycosylase
• putative methyl-CpG binding protein

Background

MBD4, methyl-CpG binding domain protein 4 (MED1), is part of the mammalian DNA glycosylase superfamily. MBD4 may be involved in maintaining genome stability, epigenetics via DNA methylation, as well as carcinogenesis. MBD4 has an amino-proximal methyl-CpG binding domain and a C-terminal mismatch-specific glycosylase domain. This configuration is thought to help maintain genome stability (PMID: 21820404). Proteins with methylcytosine-binding domains act as transcriptional repressors via promoter methylation, as well as promote the gene-silencing effect of DNA methylation. A study of patients with primary immune thrombocytopenia has shown that a decrease in expression of MBD4 may be involved with the pathogenesis of the disease (PMID: 21377502). Additionally, MBD4 has been shown to be a human DNA repair protein that is possibly involved in DNA mismatch repair. Furthermore, a study investigating microsatellite instability found that the MBD4 codon 346 polymorphism may be a factor in colorectal cancer susceptibility in the Korean population (PMID: 19469655).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBD4