MBD3 Antibody (106B691) - BSA Free

Western Blot: MBD3 Antibody (106B691) [NB100-56534] - Analysis of MBD2 and MBD3 using this antibody. HeLa cell lysate (nuclear fraction) probed with MBD3/2 antibody at 2 ug/ml in the A) absence and B) presence of immunizing peptide.

Immunohistochemistry-Paraffin: MBD3 Antibody (106B691) [NB100-56534] - MBD3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human MBD3 (106B691) Monoclonal Antibody (Catalog # NB100-56534) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in horionic villi.

Product Discontinued

MBD3 Antibody (106B691) - BSA Free Summary

• Immunogen: Between amino acids 200-250 of human MBD3 protein were used as the immunogen.
• Specificity: The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kDa and human MBD3 (291 amino acids) is 33 kDa. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBD3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 ug / million cells
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 2 ug/mL
• Control:

  HeLa Nuclear Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17546630).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for MBD3 Antibody (106B691) - BSA Free

• MBD3
• methyl-CpG binding domain protein 3
• methyl-CpG-binding domain protein 3
• Methyl-CpG-binding protein MBD3

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBD3 Antibody (NB100-56534)(3)

Publications using NB100-56534

• Ruddock-D'Cruz NT, Xue J, Wilson KJ et al. Dynamic changes in the localization of five members of the methyl binding domain (MBD) gene family during murine and bovine preimplantation embryo development. Mol Reprod Dev. 2008-01-01 [PMID: 17546630] (WB, Mouse)
  
  Details:
  IF [bovine embryos (Fig 3) and mouse embryos (Fig 4)] and for WB [bovine heart, (supplementary data)] 1. MBD1 mAb (IMG-306) 2. MBD3/MBD2 mAb (IMG-296A) 3. MBD4 pAb (IMG-285).
• Thatcher KN, Peddada S, Yasui DH, Lasalle JM. Homologous pairing of 15q11-13 imprinted domains in brain is developmentally regulated but deficient in Rett and autism samples. Hum Mol Genet. 2005-03-15 [PMID: 15689352]
  
  Details:
  ChIP (Fig 6): SH-SY5Y cells
• Balmer D, Arredondo J, Samaco RC, LaSalle JM. MECP2 mutations in Rett syndrome adversely affect lymphocyte growth, but do not affect imprinted gene expression in blood or brain. Hum Genet. 2002-06-01 [PMID: 12107440]

Review for MBD3 Antibody (NB100-56534) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 08/12/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse embryonic fibroblasts
• Species: Mouse
• Lot: 106B691

FAQs for MBD3 Antibody (NB100-56534). (Showing 1 - 1 of 1 FAQs).

1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
   • These antibodies are all purified from tissue culture supernatant.

Bioinformatics

• Gene Symbol: MBD3
• Entrez:
  • 53615(Human)
• Uniprot:
  • O95983(Human)