Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The ...read more
Immunocytochemistry/ Immunofluorescence: RbAp48 Antibody (1D11) [NB500-123] - Immunofluorescence analysis of HeLa, using RbAp48 antibody at 1:100 dilution.
Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG B. 5 ug of RbAp48 antibody. The precipitated DNA was detected by PCR with primer set ...read more
Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The ...read more
ICC/IF: Permeabilization step recommended. WB: 1:500 -1:2000 in 5% Milk/PBST at 4C overnight. Use under reducing conditions.
48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4)
Protein G purified
Alternate Names for RBBP4/RbAp48 Antibody (11G10)
CAF-1 subunit C
Chromatin assembly factor 1 subunit C
Chromatin assembly factor I p48 subunit
chromatin assembly factor/CAF-1 p48 subunit
histone-binding protein RBBP4
MSI1 protein homolog
Nucleosome-remodeling factor subunit RBAP48
retinoblastoma binding protein 4
Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
Retinoblastoma-binding protein p48
Clone 1D11 is a 48 kDa nuclear protein, first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of three subunits of CAF-1, can bind to histone H4, and is a subunit of human histone deacetylase HD1. This particular hybridoma is a fusion between BALB/c splenocytes and mouse myeloma (NS-1 cells)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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