RBBP4/RbAp48 Antibody (11G10)

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Orthogonal Strategies: Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody ...read more
Immunocytochemistry/ Immunofluorescence: RbAp48 Antibody (1D11) [NB500-123] - Immunofluorescence analysis of HeLa, using RbAp48 antibody at 1:100 dilution.
Immunohistochemistry: RbAp48 Antibody (1D11) [NB500-123]
Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG B. 5 ug of RbAp48 antibody. The precipitated DNA was detected by PCR with primer set ...read more
Orthogonal Strategies: Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody ...read more
Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - Myc-tag antibody immunoprecipitation confirmed pulldown of NuRD component RbAp48 which is known to interact with ZNF827. * denotes IgG band at 55 kDa. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Func, ICC/IF, IHC, IHC-P, IP, B/N
Clone
11G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

RBBP4/RbAp48 Antibody (11G10) Summary

Immunogen
The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
Specificity
This is specific for full length RbAp48.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RBBP4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Chromatin Immunoprecipitation
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Immunoprecipitation
  • Western Blot 1:500 - 1:3000
Application Notes
ICC/IF: Permeabilization step recommended.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB500-123 in the following applications:

Publications
Read Publications using
NB500-123 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RBBP4/RbAp48 Antibody (11G10)

  • CAF-1 p48
  • CAF-1 subunit C
  • CAF-1C
  • CAF-I p48
  • Chromatin assembly factor 1 subunit C
  • Chromatin assembly factor I p48 subunit
  • chromatin assembly factor/CAF-1 p48 subunit
  • histone-binding protein RBBP4
  • MSI1 protein homolog
  • Nucleosome-remodeling factor subunit RBAP48
  • NURF55
  • RBAP48
  • RBBP4
  • RBBP-4
  • retinoblastoma binding protein 4
  • Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
  • Retinoblastoma-binding protein p48

Background

Clone 1D11 is a 48 kDa nuclear protein, first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of three subunits of CAF-1, can bind to histone H4, and is a subunit of human histone deacetylase HD1. This particular hybridoma is a fusion between BALB/c splenocytes and mouse myeloma (NS-1 cells)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RBBP4/RbAp48 Antibody (NB500-123)(5)

Review for RBBP4/RbAp48 Antibody (NB500-123) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-123:
Filter by Applications
Immunoprecipitation
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation RBBP4/RbAp48 NB500-123
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5
reviewed by:
Verified Customer
Immunoprecipitation Human 12/18/2020
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Summary

ApplicationImmunoprecipitation
Sample TestedU-2 OS cells
SpeciesHuman
Lot30506

Comments

CommentsZNF827 was overexpressed in U-2 OS cells with a Myc-tag. Myc-tag IP was performed using CST #2276 antibody (Ms). Mouse IgG antibody was used as a control.

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WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for RBBP4/RbAp48 Antibody (NB500-123) (0)

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Verified Customer
12/18/2020
Application: Immunoprecipitation
Species: Human

Bioinformatics

Gene Symbol RBBP4
Entrez