MBD3 Antibody (106B691)

Images

 
Western Blot: MBD3 Antibody (106B691) [NB100-56534] - Analysis of MBD2 and MBD3 using this antibody. HeLa cell lysate (nuclear fraction) probed with MBD3/2 antibody at 2 ug/ml in the A) absence and B) presence of ...read more
Immunohistochemistry-Paraffin: MBD3 Antibody (106B691) [NB100-56534] - MBD3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human MBD3 (106B691) Monoclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, IHC, IHC-P, ICC/IF
Clone
106B691
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MBD3 Antibody (106B691) Summary

Immunogen
Between amino acids 200-250 of human MBD3 protein were used as the immunogen.
Specificity
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kDa and human MBD3 (291 amino acids) is 33 kDa. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MBD3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 ug / million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunocytochemistry
Control
HeLa Nuclear Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-56534 in the following applications:

Publications
Read Publications using
NB100-56534 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17546630).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MBD3 Antibody (106B691)

  • MBD3
  • methyl-CpG binding domain protein 3
  • methyl-CpG-binding domain protein 3
  • Methyl-CpG-binding protein MBD3

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
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Publications for MBD3 Antibody (NB100-56534)(3)

Review for MBD3 Antibody (NB100-56534) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56534:
Filter by Applications
ICC
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry MBD3 NB100-56534
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 08/12/2021
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Summary

ApplicationImmunocytochemistry
Sample TestedMouse embryonic fibroblasts
SpeciesMouse
Lot106B691

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for MBD3 Antibody (NB100-56534). (Showing 1 - 1 of 1 FAQs).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56534AF350
Alexa Fluor 405 NB100-56534AF405
Alexa Fluor 488 NB100-56534AF488
Alexa Fluor 532 NB100-56534AF532
Alexa Fluor 594 NB100-56534AF594
Alexa Fluor 647 NB100-56534AF647
Alexa Fluor 700 NB100-56534AF700
Alexa Fluor 750 NB100-56534AF750
Biotin NB100-56534B
DyLight 350 NB100-56534UV
DyLight 405 NB100-56534V
DyLight 488 NB100-56534G
DyLight 550 NB100-56534R
DyLight 594 NB100-56534DL594
DyLight 650 NB100-56534C
DyLight 680 NB100-56534FR
DyLight 755 NB100-56534IR
FITC NB100-56534F
HRP NB100-56534H

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Recent Reviews

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1
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Verified Customer
08/12/2021
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol MBD3
Entrez
Uniprot