Western Blot: MBD3 Antibody (106B691) [NB100-56534] - Analysis of MBD2 and MBD3 using this antibody. HeLa cell lysate (nuclear fraction) probed with MBD3/2 antibody at 2 ug/ml in the A) absence and B) presence of ...read more
Immunohistochemistry-Paraffin: MBD3 Antibody (106B691) [NB100-56534] - MBD3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human MBD3 (106B691) Monoclonal Antibody (Catalog ...read more
Between amino acids 200-250 of human MBD3 protein were used as the immunogen.
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kDa and human MBD3 (291 amino acids) is 33 kDa. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Mouse reactivity reported in scientific literature (PMID: 17546630).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for MBD3 Antibody (106B691)
methyl-CpG binding domain protein 3
methyl-CpG-binding domain protein 3
Methyl-CpG-binding protein MBD3
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MBD3 Antibody (NB100-56534). (Showing 1 - 1 of 1 FAQs).
Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
These antibodies are all purified from tissue culture supernatant.
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