MBD3 Antibody (106B691)


Western Blot: MBD3 Antibody (106B691) [NB100-56534] - Analysis of MBD2 and MBD3 using this antibody. HeLa cell lysate (nuclear fraction) probed with MBD3/2 antibody at 2 ug/ml in the A) absence and B) presence of ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC-P, ICC
0.5 mg/ml

MBD3 Antibody (106B691) Summary

Between amino acids 200-250 of human MBD3 protein were used as the immunogen.
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


  • Western Blot 2 ug/ml
  • Immunohistochemistry-Paraffin
  • Immunocytochemistry
Read Publications using
NB100-56534 in the following applications:

  • WB
    1 publication

Alternate Names for MBD3 Antibody (106B691)

  • methyl-CpG binding domain protein 3
  • methyl-CpG-binding domain protein 3
  • Methyl-CpG-binding protein MBD3


DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBD3 Antibody (NB100-56534)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MBD3 Antibody (NB100-56534). (Showing 1 - 1 of 1 FAQs).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-56534AF405
Alexa Fluor (R) 488 Labeled NB100-56534AF488
Alexa Fluor (R) 647 Labeled NB100-56534AF647
Alexa Fluor (R) 700 Labeled NB100-56534AF700
Allophycocyanin Labeled NB100-56534APC
Biotin Labeled NB100-56534B
DyLight 350 Labeled NB100-56534UV
DyLight 405 Labeled NB100-56534V
DyLight 405LS Labeled NB100-56534V3
DyLight 488 Labeled NB100-56534G
DyLight 550 Labeled NB100-56534R
DyLight 650 Labeled NB100-56534C
DyLight 680 Labeled NB100-56534FR
DyLight 755 Labeled NB100-56534IR
HRP Labeled NB100-56534H
PE Labeled NB100-56534PE
PerCP Labeled NB100-56534PCP

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Contact Information

Product PDFs


Gene Symbol MBD3

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