MAVS Antibody (981240) [Unconjugated]

MAVS was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human MAVS Monoclonal Antibody (Catalog # MAB9694) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 981240
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MAVS Antibody (981240) [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human MAVS
  Arg37-Pro513
  Accession # Q7Z434
• Specificity: Detects human MAVS in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MAVS Antibody (981240) [Unconjugated]

• CARD adapter inducing interferon beta
• CARD adapter inducing interferon-beta
• CARD adaptor inducing IFN-beta
• Cardif
• DKFZp547C224
• DKFZp666M015
• FLJ27482
• IFN-B promoter stimulator 1
• Interferon beta promoter stimulator protein 1
• interferon-beta promoter stimulator protein 1
• IPS-1
• IPS-1FLJ41962
• IPS1MGC3260
• KIAA1271FLJ35386
• MAVS
• mitochondrial antiviral signaling protein
• mitochondrial antiviral-signaling protein
• mitochondrial viral signaling protein
• Putative NF-kappa-B-activating protein 031N
• virus-induced signaling adapter variant 1b
• virus-induced signaling adaptor variant 1a
• Virus-induced-signaling adapter
• VISA
• VISAFLJ38051

Background

Mitochondrial antviral signaling protein (MAVS, also known as IPS-1, VISA, and CARDIF) is a ubiquitous, tail-anchored membrane protein inserted in the outer mitochondrial membrane (OMM), and is also found in the peroxisomal membrane and the mitochondrial associated membrane of the endoplasmic reticulum. Peroxisomal MAVS has been implicated in type II IFN-dependent antiviral response, while mitochondrial MAVS mediates longer lasting type I IFN dependent antiviral response. Mitochondrial reactive oxygen species (mROS) induce oligomerization of MAVS in the outer membrane plane of mitochondria to induce innate immunity to viral infection. MAVS undergoes RIG-I-like receptors (RLR)-induced formation of higher order structures ranging from dimers to prion-like aggregates that lead to the recruitment and activation of signaling proteins culminating in the activation of the transcription factors IRF-3 and NF-kappa B and transcription of IFNs

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MAVS Antibody (MAB9694). (Showing 1 - 1 of 1 FAQ).

1. Molecular weight of MAVS?
   • MAVS is a 540 aa protein with a predicted size of 56 kDa.

Bioinformatics

• Uniprot:
  • Q7Z434()