MAST205 Antibody (OTI3A5) - Azide and BSA Free

Western Blot: MAST205 Antibody (OTI3A5) - Azide and BSA Free [NBP2-72592] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAST2.

Western Blot: MAST205 Antibody (OTI3A5) - Azide and BSA Free [NBP2-72592] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MAST2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MAST2 monoclonal antibody. Then the blotted membrane was stripped and reprobed with

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clone: OTI3A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MAST205 Antibody (OTI3A5) - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse MAST205 Antibody (OTI3A5) - Azide and BSA Free (NBP2-45756) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1482-1798 of human MAST2(NP_055927) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAST2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:2000
• Theoretical MW: 196.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for MAST205 Antibody (OTI3A5) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• FLJ39200
• KIAA0807RP4-533D7.1
• MAST205microtubule associated testis specific serine/threonine protein kinase
• microtubule associated serine/threonine kinase 2
• microtubule-associated serine/threonine-protein kinase 2
• MTSSK

Background

MAST2 (microtubule-associated serine/threonine-protein kinase 2) is also known as MAST205. MAST2 contains a well conserved protein kinase C/A kinase domain and a PDZ domain. It is highly expressed in spermatids and has been shown to play a role in cytokine responses in macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAST2