PTEN2/TPTE Antibody

Western Blot: PTEN2/TPTE Antibody [NB100-41395] - (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PTEN2/TPTE Antibody [NB100-41395] - staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PTEN2/TPTE Antibody Summary

• Description: Novus Biologicals Goat PTEN2/TPTE Antibody (NB100-41395) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PTEN2/TPTE Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ERRTDKTHSEKFQ corresponding to internal region according to NP_954870.2, NP_954868.1, NP_954869.1.
• Specificity: This antibody is expected to recognise all reported isoforms (NP_954870.2, NP_954868.1 and NP_954869.1). It may cross-react with the almost identical TPTE2 (GeneID 93492; NP_570141.2, NP_954864.1, NP_954863.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPTE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50-55 kDa band observed in human testis lysates (calculated MW of 60.2 kDa band according to NP_954869.1).
  IHC: Paraffin embedded Human Adrenal Gland.
• Control:

  PTEN2/TPTE Overexpression Lysate

• Control Peptide:

  PTEN2/TPTE Peptide (NB100-41395PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTEN2/TPTE Antibody

• Cancer/testis antigen 44
• EC 3.1.3.48
• PTEN2
• putative tyrosine-protein phosphatase TPTE
• tensin, putative protein-tyrosine phosphatase
• transmembrane phosphatase with tensin homologytensin, putative protein-tyrosine phosphatase, EC 3.1.3.4810CT44PTEN-related tyrosine phosphatase
• Tumor antigen BJ-HCC-5

Background

TPTE(PTEN2/TPTE ) encodes a putative transmembrane tyrosine phosphatase and maps to the pericentromeric region of human chromosomes 21 and 13, and to chromosomes 15, 22, and Y. TPTE is exclusively expressed in testis. It is an integral membrane protein. It could be involved in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTEN2/TPTE Antibody (NB100-41395)(1)

Publication using NB100-41395

• Tapparel C, Reymond A, Girardet C et al. The TPTE gene family: cellular expression, subcellular localization and alternative splicing. Gene 2003-12-24 [PMID: 14659893]

Bioinformatics

• Gene Symbol: TPTE
• Entrez:
  • 7179(Human)
• Uniprot:
  • NP_954870.2(Human)