MAST1 Antibody - BSA Free

Western Blot: MAST1 Antibody [NBP2-17228] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MAST1 antibody [N2N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: MAST1 Antibody [NBP2-17228] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MAST1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MAST1 Antibody - BSA Free (NBP2-17228) is a polyclonal antibody validated for use in WB. Anti-MAST1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human MAST1. The exact sequence is proprietary.
• Localization: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasm; cytoskeleton (By similarity)
• Predicted Species: Rat (98%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAST1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 171 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MAST1 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• KIAA0973microtubule-associated serine/threonine-protein kinase 1
• microtubule associated serine/threonine kinase 1
• SAST170
• SASTsyntrophin associated serine/threonine kinase
• Syntrophin-associated serine/threonine-protein kinase

Background

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MAST1 Antibody (NBP2-17228)(3)

Publications using NBP2-17228

• Pan C, Kang J, Hwang JS Et al. Cisplatin-mediated activation of glucocorticoid receptor induces platinum resistance via MAST1 Nature communications 2021-08-16 [PMID: 34400618] (Human)
• Pan C, Chun J, Li D et al. Hsp90B enhances MAST1-mediated cisplatin resistance by protecting MAST1 from proteosomal degradation J. Clin. Invest. 2019-08-26 [PMID: 31449053] (WB, Human)
• Jin L, Chun J, Pan C et al. MAST1 Drives Cisplatin Resistance in Human Cancers by Rewiring cRaf-Independent MEk Activation. Cancer Cell. 2018-07-09 [PMID: 30033091] (WB, Human)

Bioinformatics

• Gene Symbol: MAST1
• Uniprot:
  • Q9Y2H9(Human)