![Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MAST2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MAST2 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.](http://images.novusbio.com/fullsize2/MAST205-Antibody-(OTI3A5)-Western-Blot-NBP2-45756-img0002.jpg "Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MAST2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MAST2 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, KO |
| Clone | OTI3A5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1482-1798 of human MAST2(NP_055927) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MAST2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 196.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Diseases for MAST205 Antibody (NBP2-45756)Discover more about diseases related to MAST205 Antibody (NBP2-45756).
| Pathways for MAST205 Antibody (NBP2-45756)View related products by pathway.
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PTMs for MAST205 Antibody (NBP2-45756)Learn more about PTMs related to MAST205 Antibody (NBP2-45756).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MAST2 |
![Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAST2.](http://images.novusbio.com/fullsize2/MAST205-Antibody-(OTI3A5)-Western-Blot-NBP2-45756-img0001.jpg "Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAST2.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-(MG1)-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")