Mark3 Antibody (2G12) - BSA Free

Western Blot: Mark3 Antibody (2G12) [NBP2-37445] - Analysis using MARK3 mouse mAb against HeLa (1), SK-N-SH (2), K562 (3), HCT116 (4), HEK293 (5), 3T3L1 (6), NIH3T3 (7), Jurkat (8), and A431 (9) cell lysate.

Immunohistochemistry-Paraffin: Mark3 Antibody (2G12) [NBP2-37445] - Analysis of cancer tissues using MARK3 mouse mAb with DAB staining.

Flow Cytometry: Mark3 Antibody (2G12) [NBP2-37445] - Analysis of SK-N-SH cells using MARK3 mouse mAb (green) and negative control (red).

Western Blot: Mark3 Antibody (2G12) [NBP2-37445] - Analysis using MARK3 mAb against human MARK3 recombinant protein. (Expected MW is 40.8 kDa)

ELISA: Mark3 Antibody (2G12) [NBP2-37445] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
• Clone: 2G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Mark3 Antibody (2G12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human MARK3 (AA: 435-658) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARK3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Mark3 Antibody (2G12) - BSA Free

• Cdc25C-associated protein kinase 1
• cTAK1
• C-TAK1
• CTAK1ELKL motif kinase 2
• EC 2.7.11
• EC 2.7.11.1
• EMK2
• EMK-2
• KP78
• MAP/microtubule affinity-regulating kinase 3
• PAR1A
• Protein kinase STK10
• Ser/Thr protein kinase PAR-1
• Serine/threonine-protein kinase p78

Background

MARK3 is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. MARK3 is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to MARK3, and MARK3/14-3-3 complexes are present throughout interphase but not during mitosis (1). Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (2). MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signalling, indicating a link between two important developmental pathways (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARK3