MARK4 Antibody

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Western Blot: MARK4 Antibody [NB100-1013] - (0.5ug/ml) staining of human prostate lysate (35ug protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MARK4 Antibody Summary

Immunogen
Peptide with sequence IKRKPPPGCSDSPGV corresponding to C-Terminus according to NP_113605.2.
Specificity
This antibody is expected to recognize the reported long isoform 1 (NP_113605.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MARK4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 70 kDa band observed in human prostate lysates (calculated MW of 75.3 kDa band according to NP_113605.2).
Positive Control
MARK4 Lysate (NBL1-12900)
Publications
Read Publication using NB100-1013.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MARK4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ12177
  • FLJ90097
  • KIAA1860MARK4L
  • MAP/microtubule affinity-regulating kinase 4
  • MAP/microtubule affinity-regulating kinase like 1
  • MAP/microtubule affinity-regulating kinase-like 1
  • MARK4 serine/threonine protein kinase
  • MARKL1L
  • MARKL1MARK4S
  • Nbla00650

Background

MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MARK4 Antibody (NB100-1013)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MARK4
Entrez
Uniprot