MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with NBP3-12048 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: PLK1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free (NBP3-12048) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas.
• Specificity: This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MARCO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free

• macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2
• MARCO
• member 2
• SCARA2

Background

MARCO is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram negative and Gram positive bacteria via an extracellular, C terminal, scavenger receptor cysteine rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. MARCO may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARCO