SuperBUGS in the Brain: MAP1B Antibodies & Neuronal Disease Research

Wed, 08/18/2010 - 05:03

Recently, we at Novus Biologicals added a new phospho-MAP1B (phosphorylated microtubule-associated protein 1B) antibody to our antibody database. MAP1B is a developmentally regulated phosphoprotein thought to be involved in the assembly of microtubules, an essential part of neurogenesis.

Gene knockdown antibodystudies have shown MAP1B plays an important role in the function and development of the nervous system. It may regulate microtubule function, affecting neuronal differentiation and migration, axon growth and growth-cone function. Mutated MAP1B has been implicated in a range of neural disorders including Fragile X syndrome and giant axonal neuropathy.

Antibodystudies have shown MAP1B is phosphorylated by GSK3 beta (glycogen synthase kinase 3beta) at two sites - Thr1265 and Ser1260. Phospho-MAP1B antibody (also – curiously - known as SuperBUGS) detects phosphorylated MAP1B at Thr1265.

Western Blot: MAP1B [p Thr1265] Antibody Western Blot: MAP1B [p Thr1265

Recent studies revealed phosphorylation cannot occur at the GSK3 beta site without pre-phosphorylation at a “primed site” further downstream. Primed GSK-3beta-phosphorylated MAP1B sites are found uniformly throughout the neuron, including the soma and axon. However, there are also a number of non-primed sites, which are only expressed in the axon area. These are arranged in a gradient that is highest towards the distal, or growth-cone end.

At both primed and non-primed GSK-3beta sites, phosphorylated MAP1B occurs in spatially distinctive patterns. Recently, Tymanskyj, et. al. used antibody assay techniques to investigate the evolutionary conservation of MAP1B spatial distribution in embryonic spinal cord sections.

Phospho-MAP1B antibodies raised to both primed and non-primed GSK-3beta sites in a variety of embryonic vertebrates revealed both types were remarkably conserved. However, the lowest degree of conservation occurred in non-primed sites, suggesting these evolved more recently.

Novus Biologicals offers many MAP1B reagents for your research needs including:


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