MAGP-1/MFAP2 Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MAGP-1/MFAP2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MAGP-1/MFAP2
  Gln17-Cys183
  Accession # P55002
• Specificity: Detects mouse MAGP-1/MFAP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human MAGP-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MFAP2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MAGP-1/MFAP2 Antibody

• MAGP1
• MAGP-1
• MAGP-1FLJ50901
• MAGPMFAP-2
• MFAP2
• Microfibril-associated glycoprotein 1
• microfibrillar-associated protein 2

Background

MAGP-1 (Microfibril-associated glycoprotein 1; also MFAP-2) is a 30‑33 kDa member of the MFAP family of proteins. It is secreted by endothelial and smooth muscle cells. MAGP-1 apparently mediates the release of Notch extracellular domain and also likely binds to type IV collagen and contributes to elastin fiber formation. Mature mouse MAGP-1 is 167 amino acids (aa) in length. It contains a transglutamination site at Gln20, a tyrosine sulfation motif (aa 46‑49), two potential O-linked glycosylation sites (Thr56 and Thr76), and a Cys-rich C-terminus that generates intermolecular disulfide bonds. One potential splice variant exists that is strictly intracellular and a second shows a 22 aa substitution for the C-terminal 127 amino acids. Mature mouse MAGP-1 is 94% and 87% aa identical to rat and human MAGP‑1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAGP-1/MFAP2 Antibody (AF4977)(2)

Publications using AF4977

• F Alonso, L Li, I Fremaux, DP Reinhardt, E Génot Fibrillin-1 Regulates Arteriole Integrity in the Retina Biomolecules, 2022;12(10):. 2022 [PMID: 36291539] (IHC, Mouse)
• W Jones, J Rodriguez, S Bassnett Targeted deletion of fibrillin-1 in the mouse eye results in ectopia lentis and other ocular phenotypes associated with Marfan syndrome Dis Model Mech, 2019;12(1):. 2019 [PMID: 30642872] (IHC, Mouse)

Bioinformatics

• Gene Symbol: MFAP2
• Uniprot:
  • P55002()