Fibulin 5/DANCE Antibody (293904)

Images

 
Western blot shows lysates of human heart tissue, human lung tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibulin 5/DANCE Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
293904
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Fibulin 5/DANCE Antibody (293904) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fibulin 5/DANCE
Gln24-Phe448
Accession # Q9UBX5
Specificity
Detects human Fibulin 5/DANCE in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FBLN5
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB3095 in the following applications:

Publications
Read Publications using
MAB3095 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibulin 5/DANCE Antibody (293904)

  • ARMD3fibulin-5
  • DANCE
  • DANCEFIBL-5
  • Developmental arteries and neural crest EGF-like protein
  • EVEC
  • FBLN5
  • Fibulin 5
  • UP50
  • UP50FLJ90059
  • Urine p50 protein

Background

Fibulin 5, also known as DANCE and EVEC, is a secreted 55 kDa matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis (1). Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44 amino acid (aa) spacer region, five more tandem EGF-like domains, and a 115 aa Fibulin-like C-terminal region (2, 3). Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 is expressed by smooth muscle cells and endothelial cells of the developing vasculature as well as by migrating neural crest cells and lung interstitial fibroblasts (2‑4). It is down‑regulated in the adult vasculature but is re‑expressed at aortic branching points, in the uterus, and at sites of mechanical or atherosclerotic injury (2, 3, 5). The RGD motif of Fibulin 5 binds to several cell surface Integrins including alpha V beta 3, alpha V beta 5, alpha 9 beta 1, alpha 4 beta 1, and alpha 5 beta 1 (2, 6, 7). The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix (8). Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2, and the crosslinking lysyl oxidase-like enzymes LOXL1, 2, and 4 along Fibrillin microfibrils (6, 9‑11). In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevents Fibulin 5 from interacting with Fibrillin-1 microfibrils (10). Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation and migration and by limiting vascular sprouting (5, 12). Fibulin 5 is down‑regulated in many malignancies (15). When experimentally re‑expressed, however, Fibulin 5 enhances tumor cell invasiveness and cooperates with TGF-beta in initiating and promoting epithelial-mesenchymal transition (EMT) (13, 15). Defects in Fibulin 5 expression or function can result in a loss of connective tissue integrity, cardiac elasticity, and ability to remodel the vasculature after injury (8, 5, 14).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibulin 5/DANCE Antibody (MAB3095)(4)

Review for Fibulin 5/DANCE Antibody (MAB3095) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using MAB3095:
Filter by Applications
ICC
(1)
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Filter by Species
Feline
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Fibulin 5/DANCE MAB3095
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reviewed by:
Anonymous
ICC Feline 05/23/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedAdult heart
SpeciesFeline

Product General Protocols

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Secondary Antibodies

 

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Diseases for Fibulin 5/DANCE Antibody (MAB3095)

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Recent Reviews

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5
0
4
1
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0
2
0
1
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Anonymous
05/23/2017
Application: ICC
Species: Feline

Bioinformatics

Gene Symbol FBLN5
Entrez
Uniprot