Latent TGF-beta bp2/LTBP-2 Antibody

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Western blot shows lysates of HFL1 human fetal lung fibroblast cell line and human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Latent TGF‑ beta bp2/LTBP‑2 Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Latent TGF-beta bp2/LTBP-2 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Latent TGF‑ beta  bp2/LTBP‑2
Gln36-Glu1821
Accession # Q14767
Specificity
Detects human Latent TGF‑ beta  bp2/LTBP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) LTBP-2 is observed, and less than 1% cross-reactivity with recombinant human LTBP-1 and rmLTBP-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LTBP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3850 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Latent TGF-beta bp2/LTBP-2 Antibody

  • C14orf141
  • GLC3D
  • Latent TGFbeta bp2
  • Latent TGF-beta bp2
  • latent transforming growth factor beta binding protein 2
  • latent-transforming growth factor beta-binding protein 2
  • LTBP2
  • LTBP-2
  • LTBP3chromosome 14 open reading frame 141
  • MSTP031

Background

LTBP-2 (Latent TGF-beta Binding Protein 2; also C14orf141) is a 220-250 kDa secreted glycoprotein member of the LTBP family of proteins. In SDS-PAGE and under reducing conditions, LTBP-2 runs at 220-250 kDa; under nonreducing conditions, LTBP-2 runs between 205-220 kDa. Although it is described as being a TGF-beta binding protein, it does not possess the ability to bind covalently to TGF-beta. Instead, it appears to bind to heparin sulfate moieties on syndecan-4 and perlecan, plus fibrillin-1 and fibulin-5, suggesting a role in both elastic fiber formation and the maintenance of basement membrane integrity. In addition, LTPB-2 is now known to form a complex with PC5/6A intracellularly, and upon secretion, dampen PC5 activation in the extracellular environment. Cells known to express LTBP-2 include lens and nonpigmented ciliary epithelium, fibroblasts, vascular smooth muscle and chondrocytes. Mature human LTBP-2 is 1786 amino acids (aa) in length (aa 36-1821). It possesses three heparin-binding regions (aa 94-115, 232-249, and 344-354), plus 20 EGF-like domains (aa 187-1818) that contain four interspersed TGF-beta type binding segments. Over aa 36-1821, human LTBP-2 shares 80% aa sequence identity with mouse LTBP-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Latent TGF-beta bp2/LTBP-2 Antibody (AF3850) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3850:
Filter by Applications
IHC-P
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Latent TGF-beta bp2/LTBP-2 AF3850
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reviewed by:
Andrei Turtoi
IHC-P Human 01/24/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver metastasis from colorectal tumor
SpeciesHuman
LotCHBH011611A

Comments

Comments- Antigen retrieval: Heat induced/Citrate buffer pH 6
- Primary antibody dilution: 1:50

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Recent Reviews

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1
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Andrei Turtoi
01/24/2017
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol LTBP2
Uniprot