Thimet Oligopeptidase/THOP1 Antibody (4D6)

Images

 
Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.
Immunohistochemistry: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Analysis of Thimet Oligopeptidase in mouse brain using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
4D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.54 mg/ml

Thimet Oligopeptidase/THOP1 Antibody (4D6) Summary

Background
Thimet oligopeptidase, a widely distributed enzyme in most species, plays a role in the metabolism of short neuropeptides (under 20 a.a. residues long). Thimet Oligopeptidase has an influential role in cytoplasmic peptide degradation. Other functions of Thimet Oligopeptidase include the degradation of amyloid precursor protein and the production of amyloidogenic fragments.
Immunogen
A synthetic peptide corresponding to residues 56-72 of human Thimet Oligopeptidase. [UniProt# P52888]
Localization
Cytoplasmic
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
THOP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:750-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This Thimet Oligopeptidase (4D6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~80 kDa. For IHC-P, this antibody has been tested in human, monkey, and rat brain and testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Thimet Oligopeptidase/THOP1 Lysate (NBL1-16893)
Thimet Oligopeptidase/THOP1 Lysate (NBP2-66338)
Publications
Read Publications using
NB400-146 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, mouse, rat, rabbit, and monkey.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.54 mg/ml
Purity
Protein A purified

Alternate Names for Thimet Oligopeptidase/THOP1 Antibody (4D6)

  • EC 3.4.24
  • EC 3.4.24.15
  • Endopeptidase 24.15
  • EP24.15
  • MEPD_HUMAN
  • MP78
  • thimet oligopeptidase 1
  • thimet oligopeptidase
  • THOP1
  • TOP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thimet Oligopeptidase/THOP1 Antibody (NB400-146)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Mouse
(1)
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Product General Protocols

View specific protocols for Thimet Oligopeptidase/THOP1 Antibody (NB400-146): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Thimet Oligopeptidase/THOP1 Antibody (NB400-146) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-146AF405
Alexa Fluor 488 Labeled NB400-146AF488
Alexa Fluor 647 Labeled NB400-146AF647
Alexa Fluor 700 Labeled NB400-146AF700
Biotin Labeled NB400-146B
DyLight 350 Labeled NB400-146UV
DyLight 405 Labeled NB400-146V
DyLight 488 Labeled NB400-146G
DyLight 550 Labeled NB400-146R
DyLight 650 Labeled NB400-146C
DyLight 680 Labeled NB400-146FR
DyLight 755 Labeled NB400-146IR
FITC Labeled NB400-146F
HRP Labeled NB400-146H

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Diseases for Thimet Oligopeptidase/THOP1 Antibody (NB400-146)

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Bioinformatics

Gene Symbol THOP1
Entrez
Uniprot