Thimet Oligopeptidase/THOP1 Antibody (4D6)

Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.

Immunohistochemistry: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Analysis of Thimet Oligopeptidase in mouse brain using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, ICC/IF, IHC
• Clone: 4D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.54 mg/ml

Thimet Oligopeptidase/THOP1 Antibody (4D6) Summary

• Background: Thimet oligopeptidase, a widely distributed enzyme in most species, plays a role in the metabolism of short neuropeptides (under 20 a.a. residues long). Thimet Oligopeptidase has an influential role in cytoplasmic peptide degradation. Other functions of Thimet Oligopeptidase include the degradation of amyloid precursor protein and the production of amyloidogenic fragments.
• Immunogen: A synthetic peptide corresponding to residues 56-72 of human Thimet Oligopeptidase. [UniProt# P52888]
• Localization: Cytoplasmic
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: THOP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 1:750-1:3000
• Application Notes: This Thimet Oligopeptidase (4D6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~80 kDa. For IHC-P, this antibody has been tested in human, monkey, and rat brain and testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Thimet Oligopeptidase/THOP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.54 mg/ml
• Purity: Protein A purified

Alternate Names for Thimet Oligopeptidase/THOP1 Antibody (4D6)

• EC 3.4.24
• EC 3.4.24.15
• Endopeptidase 24.15
• EP24.15
• MEPD_HUMAN
• MP78
• thimet oligopeptidase 1
• thimet oligopeptidase
• THOP1
• TOP

Background

Thimet oligopeptidase, a widely distributed enzyme in most species, plays a role in the metabolism of short neuropeptides (under 20 a.a. residues long). Thimet Oligopeptidase has an influential role in cytoplasmic peptide degradation. Other functions of Thimet Oligopeptidase include the degradation of amyloid precursor protein and the production of amyloidogenic fragments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thimet Oligopeptidase/THOP1 Antibody (NB400-146)(3)

Publications using NB400-146

• Bhatia HS, Brunner AD, OztUrk F et al. Spatial proteomics in three-dimensional intact specimens Cell 2022-12-22 [PMID: 36563667]
• Azizi AA, Kang SU, Freilinger A, Gruber-Olipitz M, Chen WQ, Yang JW, Hengstschlager M, Slavc I, Lubec G. Mitosis-dependent protein expression in neuroblastoma cell line N1E-115. J Proteome Res;7(8):3412-22. 2008-08-01 [PMID: 18582092] (WB, Mouse)
• Conn, KJ et al. Monoclonal antibodies against the human metalloprotease EC 3.4.24.15 label neurofibrillary tangles in Alzheimer's disease brain. J Neurochem. 66(5):2011-8. 1996-05-01 [PMID: 8780030]

Bioinformatics

• Gene Symbol: THOP1
• Entrez:
  • 7064(Human)
• Uniprot:
  • P52888()