Human MAG/Siglec-4a ELISA Kit (Colorimetric)

ELISA: Human MAG/Siglec-4a ELISA Kit (Colorimetric) [NBP2-81156] - Samples were spiked with high concentrations of Human MAG/Siglec-4a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human MAG/Siglec-4a ELISA Kit (Colorimetric) [NBP2-81156] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human MAG/Siglec-4a ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human MAG/Siglec-4a in samples. No significant cross-reactivity or interference between Human MAG/Siglec-4a and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.76%
• Intra-Assay: CV% < 5.15%
• Spike Recovery: 86-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MAG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MAG/Siglec-4a ELISA Kit (Colorimetric)

• GMAsialic acid binding Ig-like lectin 4A
• MAG
• myelin associated glycoprotein
• myelin-associated glycoprotein
• sialic acid-binding immunoglobulin-like lectin 4A
• Siglec4a
• Siglec-4a
• S-MAG

Background

Myelin-associated glycoprotein (MAG), a membrane glycoprotein of 100 kDa, is thought to be involved in the process of myelination (1). Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG (2). MAG has been implicated in inhibition of nerve regeneration in the CNS. This results from interactions between MAG and the Nogo receptor and gangliosides on the apposing axon, which generates intracellular inhibitory signals in the neuron (3). Results also suggest that MAG binds to a specific receptor and initiates a signal transduction cascade to effect inhibition. These results indicate that soluble dMAG detected in vivo could contribute to the lack of regeneration in the mammalian CNS after injury (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MAG