MafF Antibody [Unconjugated]

Images

 
Western blot shows recombinant human (rh) MafF, MafG, and MafK (2 ng/lane). PVDF membrane was probed with 2 µg/mL Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) followed by ...read more
Western blot shows lysates of MDA-MB-468 human breast cancer cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MafF Antigen Affinity-purified Polyclonal ...read more
MafF was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) at 15 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

MafF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MafF
Ser2-Ser164
Accession # Q9ULX9
Specificity
Detects human MafF in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) MafG and rhMafK is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAFF
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MafF Antibody [Unconjugated]

  • hMafF
  • MafF
  • transcription factor MafF
  • U-Maf
  • v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F
  • v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein F
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog F

Background

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including
c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Small Maf/Nrf2 heterodimers have been implicated in the regulation of antioxidant response element-dependent genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol MAFF
Uniprot